GE28-9575-02
TALON® Superflow™
Cytiva 28-9575-02, pack of 50 mL
About This Item
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TALON® Superflow Cobalt-IMAC
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 50 mL
manufacturer/tradename
Cytiva 28-9575-02
matrix
6% cross-linked agarose
particle size
60-160 μm
cleaning in place
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
capacity
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.
2-8°C
Related Categories
General description
Features and Benefits
- Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
- Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
Analysis Note
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Warning
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Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Protocols
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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