Skip to Content
MilliporeSigma
All Photos(1)

Documents

GE29-0485-86

HisTrap™ Excel

Cytiva 29-0485-86, pack of 1 mL

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

packaging

pack of 1 mL

manufacturer/tradename

Cytiva 29-0485-86

availability

not available in North America

parameter

1 ml/min - 4 ml/min flow rate
5 bar

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

highly cross-linked 6% agarose

average diameter

90 μm

cleaning

2-14

working range

2-13

Related Categories

General description

Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

Features and Benefits

  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • HisTrap excel columns allow direct purification of cell-free, unclarified samples
  • HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
  • HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Other Notes

H2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 1 ml/min is recommended.

Legal Information

HisTrap is a trademark of Cytiva

pictograms

Environment
GHS09

signalword

Warning

hcodes

H226

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 6

1 of 6

HisTrap™ High Performance Cytiva 17-5248-02, pack of 5 × 5 mL

GE17-5248-02

HisTrap™ High Performance

HisTrap™ High Performance Cytiva 17-5248-01, pack of 1 × 5 mL

GE17-5248-01

HisTrap™ High Performance

HiTrap® TALON® Crude Cytiva 29-0485-65, pack of 1 mL

GE29-0485-65

HiTrap® TALON® Crude

Hisprep™ Fast Flow 16/10 Cytiva 28-9365-51

GE28-9365-51

Hisprep™ Fast Flow 16/10

cOmplete™ His-Tag Purification Column Prepacked, ready-to-use chromatography column

Roche

COHISC-RO

cOmplete™ His-Tag Purification Column

Ni-NTA His•Bind® Resin

Millipore

70666

Ni-NTA His•Bind® Resin

Articles

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Protocols

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using HisTrap™ Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.

Handling Inclusion Bodies in Recombinant Protein Expression

How to isolate proteins from inclusion bodies using Cytiva products.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service