GE29-0915-98
Superose® 6 Increase
3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size
Synonym(s):
Size exclusion chromatography, Superose 6 column
About This Item
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material
glass column
product line
Superose®
mol wt
5000-5000000 Da (globular proteins)
manufacturer/tradename
Cytiva 29-0915-98
parameter
<0.03 mL/min flow rate (20% ethanol at low temp)
<0.07 mL/min flow rate (20% ethanol at room temp)
<0.07 mL/min flow rate (water at low temp)
<0.15 mL/min flow rate (water at room temp)
3 Mpa (435 psi)
4-50 μL sample volume
matrix
cross-linked agarose
polymeric
matrix active group
agarose phase
avg. part. size
8.6 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
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General description
Features and Benefits
- Increased resolution compared with Superose 6, for higher purity and improved analysis results.
- Reduced runtime compared with Superose 6, for rapid results.
- Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
- Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.
Suitability
Storage and Stability
Analysis Note
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.
Protocols
Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.
Related Content
Separation options for Size Exclusion Chromatography with Superose from Cytiva.
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