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GS500

Sigma-Aldrich

Giemsa Stain, Modified

Synonym(s):

Giemsa Solution

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About This Item

MDL number:
MFCD00081642
UNSPSC Code:
41116121
PubChem Substance ID:
24895392
NACRES:
NA.47

form

solution

Quality Level

500

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

concentration

0.4 % (w/v) in buffered methanol solution, pH 6.8, with stabilizers

pH

6.75-6.95

application(s)

hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

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General description

Giemsa stain is significantly been used to identify malarial parasite. It is is majorly employed in chromatin and nuclear membrane staining, including cytoplasm of different cell types. Giemsa stain is most prefered for staining azurophil granules over red cells and neutrophils. It also precisely stains inclusion bodies present in the smear.

Application

Giemsa stain, modified has been used in:
  • The Boyden chamber assay to study leukocyte chemotaxis.
  • Determining the number of chromosomes present in cells of newly synthesized skin tissue.
  • Chromosome aberration test.
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

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signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

target_organs

Eyes

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

51.8 °F - closed cup

flash_point_c

11 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Histophatologic Techniques (2006)
Cell Migration (2005)
Jing Zhang et al.
Cell death & disease, 11(7), 602-602 (2020-08-01)
Glioblastoma multiforme (GBM) is the most malignant primary brain tumor and has the highest mortality rate among cancers and high resistance to radiation and cytotoxic chemotherapy. Although some targeted therapies can partially inhibit oncogenic mutation-driven proliferation of GBM cells, therapies
The Giemsa stain: its history and applications
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International Journal of Surgical Pathology, 15(3) (2007)
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Monocyte/macrophage recruitment is closely associated with the degree of hypertensive renal injury. We investigated the direct role of macrophages using liposome-encapsulated clodronate (LEC) to deplete monocytes/macrophages in hypertensive renal injury. C57BL/6 mice were treated with a pressor dose of angiotensin
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