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H5912

Sigma-Aldrich

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

H2Ax Antibody, H2Ax Antibody - Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit, Anti-H2AXS139p

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About This Item

MDL number:
MFCD04974783
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 15 kDa

species reactivity

mouse, human

technique(s)

microarray: suitable
western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine

UniProt accession no.

P16104

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer139)

Gene Information

human ... H2AFX(3014)
mouse ... H2afx(15270)

General description

H2AX comprises 2-25% of the total H2A complement in human cells, whereas in the budding yeast it constitutes virtually all of the H2A molecules. It has N-terminal and C-terminal region with a central globular domain. The C-terminal region has sites for modification including acetylation, biotinylation and phosphorylation.

Immunogen

The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.
synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer139).

Application

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting.

Biochem/physiol Actions

H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA. Ataxia-telangiectasia-mutated (ATM) is the major kinase involved in the phosphorylation of H2AX in response to DNA double-strand breaks. H2AX knockout mice are radiation sensitive and show retarted growth. They also are immune deficient with mutant males are infertile..

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Loss of ATM in airway epithelial cells is associated with susceptibility to oxidative stress
Yeo AJ, et al.
American Journal of Respiratory and Critical Care Medicine, 196(3), 391-393 (2017)
H2AX: functional roles and potential applications
Dickey J, et al.
Chromosoma, 118(6), 683-692 (2009)
Genomic instability in mice lacking histone H2AX
Celeste A, et al.
Science (New York, N.Y.), 296(5569), 922-927 (2002)
Resveratrol decreases Rad51 expression and sensitizes cisplatin-resistant MCF-7 breast cancer cells
Leon-Galicia I, et al.
Oncology Reports, 39(6), 3025-3033 (2018)
Kalyan Mahapatra et al.
Scientific reports, 11(1), 11659-11659 (2021-06-04)
As like in mammalian system, the DNA damage responsive cell cycle checkpoint functions play crucial role for maintenance of genome stability in plants through repairing of damages in DNA and induction of programmed cell death or endoreduplication by extensive regulation
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