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H7006

Sigma-Aldrich

HEPES sodium salt

≥99.0% (titration)

Synonym(s):

Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
EC Number:
278-169-7
MDL number:
MFCD00036463
UNSPSC Code:
12161700
PubChem Substance ID:
24895780
NACRES:
NA.25

Quality Level

400

assay

≥99.0% (titration)

form

powder

storage condition

dry at room temperature

technique(s)

HPLC: suitable

color

white

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless

suitability

suitable for mass spectrometry

application(s)

diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics

storage temp.

room temp

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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General description

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

Application

HEPES buffer finds application in:
  • Most studies with metal ions
  • Substitute for Tris and phosphate in studies with metal ions
  • Grinding buffer in plant studies
  • Running buffer in gel electrophoresis
  • Buffer in electroporation studies
  • Bradford or bicinchoninic acid (BCA) assays
  • Extraction buffer to prevent damage of proteins in red blood cells

It has been used:
  • as a buffer component in the preparation of a low sucrose buffer
  • as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions

Features and Benefits

  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Highly soluble in water
  • Negligible metal ion binding
  • Less toxic to cells than other buffers such as Tris and phosphate
  • Stable in a wide pH range

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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