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H9289

Sigma-Aldrich

Anti-Histone H3 (N-terminal) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Histone H3 Detection, Histone H3 Detection Antibody, Rabbit Anti-Histone H3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~17 kDa

species reactivity

human

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 2-4 μg/mL using A431 cells.
western blot: 0.2-0.5 μg/mL using PC12 cell lysate and A431 cell lysate.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HIST1H3A(8350)

Related Categories

General description

Anti-Histone H3 (N-terminal) specifically recognizes human histone H3 (~17kDa). Histone H3/ histone cluster 1 H3 family member a (HIST1H3A) is a core member of the histone family. HIST1H3A is encoded by the gene mapped to human chromosome 6p22.2. The encoded protein is composed of ~136 amino acid.

Immunogen

synthetic peptide corresponding to amino acids 1-20 of human histone H3, conjugated to KLH.

Application

Anti-histone H3 (N-terminal) antibody produced in rabbit has been used in western blotting and native chromatin immunoprecipitation assay.

Biochem/physiol Actions

Histones acts as structural scaffolds for DNA packaging. Histone H3 plays an important role in replication and DNA repair. Histone H3 is heavily post-translationally modified than the other members of histone family. Mutations in the gene leads to cerebellar high-grade gliomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Histone H3 K27M mutations in adult cerebellar high-grade gliomas.
Nakata S, et al.
Brain Tumor Pathology, 34, 113-119 (2017)
Hua Jiang et al.
Genes & development, 31(12), 1272-1287 (2017-07-27)
Hybrid seed lethality as a consequence of interspecies or interploidy hybridizations is a major mechanism of reproductive isolation in plants. This mechanism is manifested in the endosperm, a dosage-sensitive tissue supporting embryo growth. Deregulated expression of imprinted genes such as
Kerstin Müller et al.
PloS one, 7(12), e51532-e51532 (2012-12-15)
Plants have a remarkable ability to react to seasonal changes by synchronizing life-cycle transitions with environmental conditions. We addressed the question of how transcriptional re-programming occurs in response to an environmental cue that triggers the major life cycle transition from
Phosphorylation of histone H3 Ser10 establishes a hierarchy
for subsequent intramolecular modification events
Liokatis S, et al.
Nature Structural and Molecular Biology, 19, 819-823 (2012)
H3K9me3-binding proteins are dispensable for SETDB1/H3K9me3-dependent retroviral silencing
Maksakova IA, et al.
Epigenetics & Chromatin, 4 (2011)
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