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H9411

Sigma-Aldrich

Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-AHO3, Anti-BDMR, Anti-HA6116, Anti-HD4, Anti-HDAC-4, Anti-HDAC-A, Anti-HDACA

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About This Item

MDL number:
MFCD03791601
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~140 kDa (additional weaker bands may be present)

species reactivity

rat, human, mouse

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunoprecipitation (IP): 10-20 μg using RIPA extract of HeLa nuclei
indirect immunofluorescence: 1:250 using HeLa cells
microarray: suitable
western blot: 1:1,000 using whole extracts of mouse NIH3T3 cells
western blot: 1:500 using whole extracts of rat brain

UniProt accession no.

P56524

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)

Related Categories

General description

Mammalian histone deacetylases (HDACs) can be divided into three classes according to sequence homology. Class II consists of the yeast Hda1-like proteins histone deacetylase 4,5,6,7,8,9, 10 (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9, and HDAC10.

Immunogen

synthetic peptide corresponding to amino acid residues 1-19 of human HDAC4 with C-terminal added lysine conjugated to KLH.

Application

Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence
  • immunostaining
  • immunohistochemistry
  • chromatin immunoprecipitation

Biochem/physiol Actions

Histone deacetylase 4 (HDAC4) exhibits intrinsic nuclear import and export signals for its dynamic nucleocytoplasmic shuttling. Its association with 14-3-3 and monocyte enhancer factor 2 (MEF2) proteins can disturb shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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flash_point_c

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Enzymatic activity associated with class II HDACs is dependent on a multiprotein complex containing HDAC3 and SMRT/N-CoR
Fischle W, et al.
Molecular Cell, 9(1), 45-57 (2002)
Lilian Varricchio et al.
The international journal of biochemistry & cell biology, 50, 112-122 (2014-03-07)
To clarify the role of HDACs in erythropoiesis, expression, activity and function of class I (HDAC1, HDAC2, HDAC3) and class IIa (HDAC4, HDAC5) HDACs during in vitro maturation of human erythroblasts were compared. During erythroid maturation, expression of HDAC1, HDAC2
HDAC4 governs a transcriptional program essential for synaptic plasticity and memory
Sando III R, et al.
Cell, 151(4), 821-834 (2012)
Stress-induced epigenetic transcriptional memory of acetylcholinesterase by HDAC4
Sailaja BS, et al.
Proceedings of the National Academy of Sciences of the USA, 109(52), E3687-E3695 (2012)
Histone deacetylase inhibitors preserve white matter structure and function during ischemia by conserving ATP and reducing excitotoxicity
Baltan S, et al.
The Journal of Neuroscience, 31(11), 3990-3999 (2011)
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