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I5635

Sigma-Aldrich

Monoclonal Anti-Human IgG2 antibody produced in mouse

clone HP-6014, ascites fluid

Synonym(s):

Monoclonal Anti-Human IgG2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6014, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:5,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Monoclonal Anti-Human IgG2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses.

Specificity

The antibody is specific for human IgG2 and is non-reactive with other IgG subclasses. This clone has been singled out as the most widely applicable IgG2 specific monoclonal antibody by the IUIS/WHO study.

Application

Monoclonal Anti-Human IgG2 antibody has been used in in enzyme linked immunosorbent assay (ELISA) and in western blotting.
It may also be used:
  • in imprint immunofixation
  • in immunofluorometric assay
  • in hemagglutination
  • in hemagglutination inhibition
  • in particle counting immunoassay
  • in detection of cytoplasmic IgG

Biochem/physiol Actions

Anti-polysaccharide responses are mainly of the IgG2 sub-class while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccharides stimulate an IgG2 response in peripheral blood leukocytes (PBL′s).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A conserved Leishmania hypothetical protein evaluated for the serodiagnosis of canine and human visceral and tegumentary leishmaniasis, as well as a serological marker for the posttreatment patient follow-up
Ribeiro P A, et al.
Diagnostic Microbiology and Infectious Disease, 92(3), 196-203 (2018)
Identification of immune biomarkers related to disease progression and treatment efficacy in human visceral leishmaniasis
Portela A S B, et al.
Immunobiology, 223(3), 303-309 (2018)
Negative immunodiffusion test results obtained with sera of paracoccidioidomycosis patients may be related to low-avidity immunoglobulin G2 antibodies directed against carbohydrate epitopes
Neves AR, et al.
Clinical and Diagnostic Laboratory Immunology, 10(5), 802-807 (2003)
D Thiagarajan et al.
ACR open rheumatology, 2(6), 344-356 (2020-05-12)
Immunoglobulin M antibodies against phosphorylcholine (anti-PCs) may be protective in atherosclerosis, cardiovascular disease (CVD), and systemic lupus erythematosus (SLE). We study immunoglobulin G1 (IgG1) and immunoglobulin G2 (IgG2) anti-PCs, with a focus on atherosclerosis and SLE. We determined anti-PCs by
A M Walker et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(8), 3278-3282 (1995-04-11)
Several lines of evidence indicate that immunoglobulin-bound prolactin found in human serum is not a conventional complex between an anti-prolactin antibody and prolactin but a different type of association of prolactin with the Fab portion of IgG heavy chains. The
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Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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