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I6760

Sigma-Aldrich

Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse

clone HP-6017, ascites fluid

Synonym(s):

Monoclonal Anti-Human IgG (Fc specific), Anti Human Igg Monoclonal Antibody, Anti-Human Fc Monoclonal Antibody, Anti-Human Fc Monoclonal Antibody - Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse, Mouse Anti-Human Igg

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6017, monoclonal

contains

15 mM sodium azide

species reactivity

rabbit, sheep, horse (IgG), goat, human

technique(s)

indirect ELISA: 1:3,000

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Monoclonal Anti-Human IgG (mouse IgG2a isotype) is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins (Igs) belongs immunoglobulin super-family and have two heavy (H) and two light (L) chain, held together by disulphide linkages. The four classes of IgG can be IgG1, IgG2, IgG3 and IgG4. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

Specificity

The antibody is specific for the Fc portion of human IgG and recognizes an epitope common to all human IgG subclasses. This antibody was adopted as an Fc specific reagent in the IUIS/WHO study.

Application

Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • imprint immunofixation (IIF)
  • immunofluorometric assay (IFMA)
  • hemagglutination (HA)
  • hemagglutination inhibition (HAI)
  • particle counting immunoassay(PACIA)

Biochem/physiol Actions

Although the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. A certain population of lymphocytes also possess a "Fc receptor". These functions indicate the importance of immunoreagents specific for the Fc fragment of human IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin
Vossenaar ER, et al.
Arthritis Research & Therapy, 6(2), R142-R142 (2004)
Ratio of serum IgG3 to total IgG concentration and goiter size are independent factors in intractability of Graves' disease
Santoh T, et al.
Endocrine Journal, 0711080013-0711080013 (2007)
Effector-attenuating substitutions that maintain antibody stability and reduce toxicity in mice
Lo M, et al.
The Journal of Biological Chemistry, 292(9), 3900-3908 (2017)
Structure and function of immunoglobulins
Schroeder Jr HW and Cavacini L
The Journal of Allergy and Clinical Immunology, 125, S41-S52 (2010)
S Langy et al.
Infection and immunity, 66(6), 2854-2858 (1998-05-29)
The full-length cDNA sequence encoding Brugia malayi L3 paramyosin has been isolated by immunoscreening a cDNA library with a mouse antiserum raised against Wuchereria bancrofti L3 infective larvae. A recombinant truncated form of paramyosin was expressed as a glutathione S-transferase
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