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K0265

Sigma-Aldrich

Anti-Potassium Channel K2P4.1 (TRAAK) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-KCNK4, Anti-TWIK-related arachidonic acid-stimulated K+ channel

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About This Item

MDL number:
MFCD09753858
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human, rat

technique(s)

immunohistochemistry: suitable
western blot: 1:200 using HEK-K2P4.1 transfected cells or rat cerebellum lysate

UniProt accession no.

Q9NYG8

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KCNK4(50801)
rat ... Kcnk4(116489)

General description

The gene K2P4.1, also referred to as KCNK4 (potassium two pore domain channel subfamily K member 4), encodes a protein belonging to the subfamily of K2P (two pore domain K+ channel) channels called TRAAK (TWIK-related arachidonic acid-stimulated K+ channel). The TRAAK channels are expressed selectively in the nervous system. TRAAK channel contains two protomers, each with two distinct pore domains forming a two-fold symmetric potassium channel.

Specificity

The K2P4.1 antibody is directed against a highly conserved epitope in the intraracellular C-terminal region of the human K2P4.1 channel.

Immunogen

peptide NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human K2P4.1.

Biochem/physiol Actions

The gene KCNK4 (potassium two pore domain channel subfamily K member 4) encodes a potassium channel that is mechanosensitive and is regulated by polyunsaturated fatty acids and temperature. The structure and chemical composition of the lipid bilayer has a profound effect on the functioning of this K+ channel. It is found to regulate the noxious input threshold in dorsal root ganglia neurons.

Physical form

Lyophilized powder from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.025% sodium azide.

Reconstitution

Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein, such as BSA (1-3%).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Trichloroethanol enhances the activity of recombinant human TREK-1 and TRAAK channels.
Harinath S and Sikdar SK
Neuropharmacology, 46, 750-760 (2004)
Crystal structure of the human K2P TRAAK, a lipid- and mechano-sensitive K+ ion channel.
Brohawn SG
Science, 335, 436-441 (2012)

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