L0280
Latex beads, amine-modified polystyrene, fluorescent blue
aqueous suspension, 2.0 μm mean particle size
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form
aqueous suspension
Quality Level
composition
Solids, 2.5%
technique(s)
cell based assay: suitable
mean particle size
2.0 μm
fluorescence
λex ~360 nm; λem ~420 nm
application(s)
cell analysis
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Application
Latex beads, amine-modified polystyrene, fluorescent blue has been used:
- as inert beads to study the role of autophagy proteins in LC3-associated phagosome (LAP) maturation
- in the culturing of peritoneal cells for phagocytic latex bead assay ex vivo
- in the preparation of LAP stimuli coated beads
Biochem/physiol Actions
Polystyrene latex beads can be used to create latex agglutination systems. Polystyrene latex beads have been used to study the transmission of Mycobacterium leprae, the causative pathogen of leprosy, as well as to develop a method for mass screening for both pulmonary and extrapulmonary tuberculosis.
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Biology of reproduction, 60(3), 747-755 (1999-02-20)
To initiate normal embryonic development, an egg must receive a signal to become activated at fertilization. We here report that the ability of demembranated sperm heads to activate is abolished after incubation over the range 20-44 degreesC and is sensitive
Journal of Alzheimer's disease : JAD, 14(3), 345-352 (2008-07-05)
Microglial phagocytosis of amyloid-beta (Abeta) deposits is involved in Abeta clearance in vivo. To explore the ability of microglia to phagocytose beta, we cultured human microglia or human monocytic THP-1 cells directly on unfixed frontal cortex sections of an Alzheimer
Methods in molecular biology (Clifton, N.J.), 1519, 145-168 (2016-11-07)
The noncanonical macroautophagy pathway, LC3-associated phagocytosis (LAP) has recently emerged as an important catabolic process involved during exogenous antigen processing. It has been described that in human macrophages and dendritic cells the direct recruitment of LC3 to the phagosomal membrane
Journal of dermatological science, 46(2), 101-110 (2007-02-24)
The transmission of Mycobacterium leprae, the causative pathogen of leprosy, has been postulated to occur mainly through upper respiratory route rather than skin-to-skin contact via minor injuries. The M. leprae genome contains mce1A gene, which encodes a putative mammalian cell
Journal of immunoassay & immunochemistry, 23(3), 399-406 (2002-09-14)
Polystyrene latex beads were polymerized at two different pH (7.3 and 9.5) and their ability to create latex agglutination systems were studied. Sensitization of these latexes by DNA, antibodies to C-reactive protein, myoglobin, and human IgG. was conducted. Sensitivity and
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