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L3272

Sigma-Aldrich

LB Broth with agar (Luria low salt)

Powder microbial growth medium

Synonym(s):

Luria Agar Base (Miller’s Modification)

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About This Item

UNSPSC Code:
12352200

grade

for molecular biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(3.05% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Application

LB Broth with agar (Luria low salt) has been used:
  • for culturing E. coli DH10B cells.
  • in the isolation of diazotrophic bacteria.
  • for transformation of competent E. coli strain Stbl3 for cloning.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Luria′s low salt LB powder with agar provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to pre-poured plates
  • Standard formulation

Preparation Note

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Difco Manual, 271-271 (1998)
Increased Expression of a Reporter Plasmid by Crotalid Venom Fraction
Islas AA, et al.
Journal of Animal and Veterinary Advances, 9(17), 2214-2219 (2010)
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Grobelak A, et al.
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Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes
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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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