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L6876

Sigma-Aldrich

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Synonym(s):

Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

CAS Number:
12650-88-3
Enzyme Commission number:
3.2.1.17 (BRENDA, IUBMB)
EC Number:
235-747-3
MDL number:
MFCD00131557
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

chicken egg white

Quality Level

300

form

lyophilized powder

specific activity

≥40,000 units/mg protein

mol wt

single-chain 14.3 kDa

composition

protein, ≥90%

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

UniProt accession no.

P00698

application(s)

cell analysis

shipped in

wet ice

storage temp.

−20°C

Gene Information

chicken ... LYZ(396218)

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Application

Lysozyme from chicken egg white has been used for the extraction of genomic DNA from bacterial cells. It has been used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Features and Benefits

Lysozyme from chicken egg white is a versatile enzyme with unique properties that make it effective for various applications, including bacterial cell wall breakdown, genomic DNA extraction, and external standard for mass analysis. It is reliable, active over a broad pH range, and available in various packaging sizes.

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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substrate

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Inhibition of lung tumor colonization and cell migration with the disintegrin crotatroxin 2 isolated from the venom of Crotalus atrox.
Galan JA et al.
Toxicon, 51, 1186-1186 (2008)
Daniel W Horn et al.
Colloids and surfaces. B, Biointerfaces, 139, 237-243 (2016-01-02)
The dispersion of single-walled carbon nanotubes (SWNT) in aqueous solutions of biological materials enables the production of bulk films and fibers that combine natural biological activity with SWNT's intrinsic mechanical, thermal, and electrical properties. In this work, we report the
Sibel Türkkan et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 106(7), 2625-2635 (2018-01-24)
In this study, novel porous three-dimensional (3D) scaffolds from silk fibroin (SF) and functionalized (amidated and oxidized) citrus pectin (PEC) were developed for skin tissue engineering applications. Crosslinking was achieved by Schiff's reaction in borax presence as crosslinking coordinating agent
Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer.
Chen W et al.
PLoS ONE, 7, e39743-e39743 (2012)
A rapid procedure for isolating chromosomal DNA from Lactobacillus species and other Gram-positive bacteria.
Ulrich RL and Hughes TA
Letters in Applied Microbiology, 32, 52-52 (2001)
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Protocols

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

Chromatograms

application for HPLC

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