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L8647

Sigma-Aldrich

Levan from Erwinia herbicola

≥98% (TLC)

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About This Item

CAS Number:
9013-95-0
MDL number:
MFCD00131502
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

bacterial (Erwinia herbicola)

Quality Level

200

assay

≥98% (TLC)

form

powder

technique(s)

thin layer chromatography (TLC): suitable

color

white

solubility

water: 9.80-10.20 mg/mL, cloudy to hazy, colorless to faintly yellow

storage temp.

2-8°C

InChI

1S/C18H32O16/c19-1-7-10(23)14(27)17(5-21,33-7)31-3-9-12(25)15(28)18(6-22,34-9)30-2-8-11(24)13(26)16(29,4-20)32-8/h7-15,19-29H,1-6H2/t7-,8-,9-,10-,11-,12-,13+,14+,15+,16-,17-,18-/m1/s1

InChI key

ZFTFOHBYVDOAMH-XNOIKFDKSA-N

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General description

Levan is a polysaccharide produced by some microorganisms and plants. It is a non-typical biopolymer composed of fructose units linked by β-(2→6). Levan is an exopolysaccharide (synthesized outside the cell).

Application

Levan from Erwinia herbicola has been used as a:
  • standard for the nuclear magnetic resonance (NMR) spectroscopy analysis of levan produced by Bacillus licheniformis NS032 in sugar beet molasses-based medium
  • polysaccharide substrate to test the ability of Prevotella copri isolates to grow when polysaccharides were provided as the sole carbon source in vitro
  • substrate in levanase activity assay

Biochem/physiol Actions

Levan has various industrial applications like cosmetics, medicine, or nanotechnology. It might exhibit anti-hyperglycemic effect and anti-tumor action, prevent diabetes-induced oxidative stress and increase catalase (CAT) and superoxide dismutase (SOD) in the heart. Levan is also recognized for its immunomodulating and prebiotic actions.

Other Notes

A polymer of β-D-fructose
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Susanne L Jensen et al.
International journal of biological macromolecules, 85, 514-521 (2016-01-17)
A Levanase, LevB, from Bacillus subtilis 168, was expressed as a His6-tagged protein in Escherichia coli. The enzyme was purified and characterised for its activity and substrate specificity. LevB has a pH optimum of 6.0-6.5 and a maximum observed specific
Payal Joglekar et al.
mSphere, 3(3) (2018-05-26)
Genomic differences between gut-resident bacterial strains likely underlie significant interindividual variation in microbiome function. Traditional methods of determining community composition, such as 16S rRNA gene amplicon sequencing, fail to capture this functional diversity. Metagenomic approaches are a significant step forward
G D Gojgic-Cvijovic et al.
International journal of biological macromolecules, 121, 142-151 (2018-10-07)
The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L
Faruk Küçükaşik et al.
Applied microbiology and biotechnology, 89(6), 1729-1740 (2010-12-17)
Levan is a homopolymer of fructose with many outstanding properties like high solubility in oil and water, strong adhesiveness, good biocompatibility, and film-forming ability. However, its industrial use has long been hampered by costly production processes which rely on mesophilic
Mária Matulová et al.
Carbohydrate research, 346(4), 501-507 (2011-02-05)
Bacillus sp. 3B6, bacterium isolated from cloud water, was incubated on sucrose for exopolysaccharide production. Dialysis of the obtained mixture (MWCO 500) afforded dialyzate (DIM) and retentate (RIM). Both were separated by size exclusion chromatography. RIM afforded eight fractions: levan
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