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M042080

Sigma-Aldrich

Biotin Phosphoramidite

configured for PerkinElmer, configured for Polygen

Synonym(s):

Biotin amidite

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About This Item

Empirical Formula (Hill Notation):
C46H64N5O8PS
CAS Number:
1217500-22-5
Molecular Weight:
878.07
MDL number:
MFCD00676535
UNSPSC Code:
41116105
PubChem Substance ID:
329817781
NACRES:
NA.21

product line

Proligo Reagents

Quality Level

200

assay

≥96% (31P-NMR)

suitability

conforms to structure for H-NMR

compatibility

configured for PerkinElmer
configured for Polygen

storage temp.

−20°C

SMILES string

COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5

InChI

1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1

InChI key

WSDQYLGSIUJJRU-ZMKGMYJVSA-N

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General description

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis.

The photocleavable biotin phosphoramidite reagent is compatible with automated DNA/RNA synthesizers using phosphoramidite chemistry. The length of the p-aminophenethyl spacer group allows streptavidin affinity purification of the oligonucleotide by reverse-phase HPLC. Biotinlabeling is compatible with PCR and most hybridization techniques.Key Features of Biotin Phosphoramidite
  • Behaves like any standard phosphoramidite on the DNA synthesizer
  • Readily soluble in acetonitrile
  • Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification• A long, water-compatible linker arm ensures maximum sensitivity
  • Provides a coupling efficiency of at least 95%The product is configured for Expedite™ and PolyGen® Synthesizers.

Application

Biotin Phosphoramidite is suitable for the synthesis of 5′-biotinylated oligonucleotides whose applications are seen in ligase-mediated gene detection, direct dideoxy sequencing following PCR, and non-radioactive sequencing of DNA.

Legal Information

Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
PolyGen is a registered trademark of PolyGen GmbH.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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