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M1821

Sigma-Aldrich

Anti-Monoamine Oxidase B antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Adrenalin oxidase, Anti-MAO, brain, Anti-MAO, platelet, Anti-Tyramine oxidase

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

mouse, rat, human

concentration

~1.5 mg/mL

technique(s)

western blot: 0.5-1.0 μg/mL using rat liver mitochondrial fraction

UniProt accession no.

P27338

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ASCC3(10973) , MAOB(4129)
rat ... Maob(25750)

General description

Monoamine Oxidase B (MAO-B) is a mitochondrial flavo enzyme. It is mainly found in the brain in dopaminergic, serotonergic, and histaminergic neurons and astrocytes.
Monoamine Oxidase B is encoded by the gene mapped to human chromosome Xp11.3.

Specificity

Anti-Monoamine Oxidase B specifically recognizes human, mouse, and rat MAO-B.

Application

Anti-Monoamine Oxidase B antibody has been used in western blotting.
Anti-Monoamine Oxidase B antibody produced in rabbit has also been used in coimmunoprecipitation (co-IP) and immunohistochemistry.

Biochem/physiol Actions

Monoamine oxidase B (MAO-B) is considered the predominant isoform responsible for dopamine metabolism in the human central nervous system (CNS). MAO-B activity correlates with personality traits. Alterations in MAO-B activity may underlie dopamine pathobiology in major depression. Selective MAO-B inhibitors elevate synaptosomal dopamine concentrations, and have recently shown clinical efficacy in the treatment of early Parkinson′s disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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Immunocytochemical demonstration of monoamine oxidase B in brain astrocytes and serotonergic neurons
Levitt P, et al.
Proceedings of the National Academy of Sciences of the USA, 79(20), 6385-6389 (1982)
Rasagiline [N-propargyl-1R (+)-aminoindan], a selective and potent inhibitor of mitochondrial monoamine oxidase B
Youdim M, et al.
British Journal of Pharmacology, 132(2), 500-506 (2001)
Structure of human monoamine oxidase B, a drug target for the treatment of neurological disorders
Binda C, et al.
Nature Structural and Molecular Biology, 9(1), 22-22 (2002)
Dorit Trudler et al.
Journal of neurochemistry, 129(3), 434-447 (2013-12-21)
DJ-1 is an oxidative stress sensor that localizes to the mitochondria when the cell is exposed to oxidative stress. DJ-1 mutations that result in gene deficiency are linked to increased risk of Parkinson's disease (PD). Activation of microglial stress conditions
Sophia Schedin-Weiss et al.
Alzheimer's research & therapy, 9(1), 57-57 (2017-08-03)
Increased levels of the pathogenic amyloid β-peptide (Aβ), released from its precursor by the transmembrane protease γ-secretase, are found in Alzheimer disease (AD) brains. Interestingly, monoamine oxidase B (MAO-B) activity is also increased in AD brain, but its role in
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