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M3148

Sigma-Aldrich

2-Mercaptoethanol

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
Beilstein/REAXYS Number:
773648
EC Number:
200-464-6
MDL number:
MFCD00004890
UNSPSC Code:
12161504
PubChem Substance ID:
24896800
NACRES:
NA.31

grade

for molecular biology

Quality Level

200

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

product line

BioReagent

assay

99% (GC/titration)

form

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

concentration

14.3 M (pure liquid)

technique(s)

cell culture | mammalian: suitable
electrophoresis: suitable

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: soluble 1 mL/mL

density

1.114 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, protease, none detected

storage temp.

room temp

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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Related Categories

Application

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
  • as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
  • to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
  • to trypsinize mouse embryonic stem cells
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Storage Class

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

154.4 °F - closed cup

flash_point_c

68 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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