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M5062

Sigma-Aldrich

Anti-Myosin Va (LE-16) antibody produced in rabbit

enhanced validation

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
MFCD03455612
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 190 kDa

species reactivity

rat, chicken

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~0.5 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using rat and chicken cerebellum sections
microarray: suitable
western blot: 1:500 using a rat brain extract

UniProt accession no.

Q02440

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYO5A(4644)
mouse ... Myo5a(17918)
rat ... Myo5a(25017)

Related Categories

General description

Myosin Va (p190) is a member of the unconventional class of myosins, distinct from both the myosins I and myosin II. It is present in neuronal and non neuronal cells of the brain. Class V myosins are widely expressed actin-based motors. Class V myosins have two motor head domains typical of myosins, and an extended regulatory neck domain with six tandem IQ domains that bind multiple calmodulin light chains. In addition, myosin V contains a unique 400 amino acids globular tail domain that may direct myosin V to its target or determine the cargo to which it binds.

Immunogen

synthetic peptide located near the C-terminus of chicken myosin Va (amino acids 1705-1720 with N-terminally added lysine) conjugated to KLH. This sequence is identical in human, mouse and rat.

Application

Anti-Myosin Va (LE-16) antibody produced in rabbit is used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Myosin Va is implicated in the regulation of vesicle trafficking in neurons and melanocytes. It regulates melanosome distribution along microfilaments. It is found in association with the centrosome at all stages of the cell cycle. In the interphase stage, myosin Va is found in pericentriolar material. During cell division, it is found in the cytoplasm and concentrates in a trail between migrating centrioles and in the mitotic spindle poles and spindle fibers.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regulated conformation of myosin V
Wang F, et al.
The Journal of Biological Chemistry, 279(4), 2333-2336 (2004)
Katharina N Richter et al.
Scientific reports, 8(1), 14838-14838 (2018-10-06)
Protein copy numbers can be measured by biochemical methods ranging from quantitative Western Blotting to several mass spectrometry approaches. Such methods only provide average copy numbers, obtained over large cell numbers. However, copy number estimates for single cells or single
A millennial myosin census
Berg JS, et al.
Molecular Biology of the Cell, 12(4), 780-794 (2001)
High affinity binding of brain myosin-Va to F-actin induced by calcium in the presence of ATP
Tauhata SBF, et al.
The Journal of Biological Chemistry, 276(43), 39812-39818 (2001)

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