M6065
Monoclonal Anti-Microphthalmia antibody produced in mouse
clone C5, purified immunoglobulin
Synonym(s):
Anti-Mi
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
C5, monoclonal
form
buffered aqueous solution
mol wt
antigen 52-56 kDa
species reactivity
mouse, rat, human
concentration
0.5-1.0 mg/mL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable using 2 μg/mg protein lysate
western blot: 1:500 using 10 μg of Mouse brain lysates
isotype
IgG1
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MITF(4286)
mouse ... Mitf(17342)
rat ... Mitf(25094)
General description
Microphthalmia (Mi in mouse or MITF in human) is a basic helix-loop-helix-leucine zipper (BHLH–ZIP) transcription factor involved in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium, i.e. cells responsible for hair, skin, and eye color. It activates the expression of the melanocyte specific genes tyrosinase and TRP1 (tyrosinase-related protein 1) by binding as a homo- or heterodimer to a symmetrical DNA sequence (E box) (5′-CATGTG-3′) located within the M box found in their promoters. Microphthalmia also appears to be involved in the differentiation of mast cells, osteoclasts, basophils and natural killer cells.
Microphthalmia is expressed in a limited number of cell types including heart, mast cells, osteoclast precursors, and melanocytes. There are a number of different isoforms of microphthalmia resulting from alternative splicing and alternative promotors. These isoforms differ at their amino-termini and in their expression patterns.
Microphthalmia is expressed in a limited number of cell types including heart, mast cells, osteoclast precursors, and melanocytes. There are a number of different isoforms of microphthalmia resulting from alternative splicing and alternative promotors. These isoforms differ at their amino-termini and in their expression patterns.
Mouse monoclonal clone C5 anti-Microphthalmia antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia from human, mouse or rat.
Immunogen
N-terminal fragment of human microphthalmia protein.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Mouse monoclonal clone C5 anti-Microphthalmia antibody is used to tag serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia for detection and quantitation by techniques such as immunoblotting (doublet of 52-56 kDa), immunoprecipitation, immunohistochemistry on formalin-fixed paraffin embedded or frozen tissue sections, and gel shift. It is used as a probe to determine the roles of microphthalmia in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium.
Physical form
Solution in phosphate buffered saline containing 0.08% sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Miguel F Segura et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(6), 1814-1819 (2009-02-04)
The highly aggressive character of melanoma makes it an excellent model for probing the mechanisms underlying metastasis, which remains one of the most difficult challenges in treating cancer. We find that miR-182, member of a miRNA cluster in a chromosomal
Natalia Pieper et al.
Oncoimmunology, 7(8), e1450127-e1450127 (2018-09-18)
The profound but frequently transient clinical responses to BRAFV600 inhibitor (BRAFi) treatment in melanoma emphasize the need for combinatorial therapies. Multiple clinical trials combining BRAFi and immunotherapy are under way to further enhance therapeutic responses. However, to which extent BRAFV600
Xueping Wang et al.
PloS one, 10(11), e0143142-e0143142 (2015-11-19)
TYR, DCT and MITF are three important genes involved in maintaining the mature phenotype and producing melanin; they therefore participate in neural crest cell development into melanocytes. Previous studies have revealed that the Wnt signaling factor lymphoid enhancer-binding factor (LEF-1)
Li-Ping Liu et al.
Cell reports, 27(2), 455-466 (2019-04-11)
Induced pluripotent stem cells (iPSCs) are a promising melanocyte source as they propagate indefinitely and can be established from patients. However, the in vivo functions of human iPSC-derived melanocytes (hiMels) remain unknown. Here, we generated hiMels from vitiligo patients using a three-dimensional
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