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M6570

Sigma-Aldrich

Anti-Melan-A antibody, Mouse monoclonal

clone A103, purified from hybridoma cell culture

Synonym(s):

Anti-MART1

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About This Item

MDL number:
MFCD01633630
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

A103, monoclonal

form

buffered aqueous solution

mol wt

antigen ~22 kDa

species reactivity

human, canine

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of SKMEL19 cells ()

isotype

IgG1

UniProt accession no.

Q16655

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MLANA(2315)

General description

Melan-A is also known as MART-1 (melanoma antigen recognized by T-cells 1). It is a transmembrane protein which is hydrophobic in nature. Melan-A is expressed in melanocytes and melanoma tumors.
Monoclonal Anti-Melan-A (mouse IgG1 isotype) is derived from the hybridoma A103 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with a human recombinant Melan-A protein. Melan-A (also known as MART1) is a melanoma antigen recognized by T cells. The protein is expressed mainly in cells and tumors of melanocytic lineage and thus belongs to the melanocyte differentiation markers.

Immunogen

recombinant human Melan-A protein.

Application

Anti-Melan-A antibody, Mouse monoclonal has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation
  • immunohistochemistry

Biochem/physiol Actions

Melan-A/MART1 may have a role in pigmentation since its expression pattern and sub cellular distribution is similar to other melanosomal proteins and correlates with melanin content. Melan-A is important for Pmel17 activity, forms a complex with it, and affects its expression, stability, trafficking, and processing that is required for melanosome structure and maturation. Thus, Melan-A is important for Pmel17 function and therefore it is important for mammalian pigmentation.

Target description

Melan-A is a melanoma antigen recognized by T cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Enayat Attwa et al.
International journal of dermatology, 54(2), 235-244 (2014-10-01)
Melasma is a common pigmentary disorder that remains resistant to available therapies. The aim of the present study was to evaluate the efficacy of erbium:YAG lasers in the treatment of refractory melasma and investigate the histopathological and ultrastructural changes between
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Immunology for Pharmacy, 181-188 (2012)
A A Jungbluth et al.
Virchows Archiv : an international journal of pathology, 434(5), 429-435 (1999-07-02)
Angiomyolipomas are tumours of uncertain histogenesis, most often occurring in association with the kidney. A characteristic finding is their reactivity with HMB-45, a monoclonal antibody to the melanocyte-associated antigen gp-100. We tested 18 angiomyolipomas for their reactivity with A103, a
Y T Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(12), 5915-5919 (1996-06-11)
Recent progress in the structural identification of human melanoma antigens recognized by autologous cytotoxic T cells has led to the recognition of a new melanocyte differentiation antigen, Melan-A(MART-1). To determine the properties of the Melan-A gene product, Melan-A recombinant protein
Lin Hou et al.
Theranostics, 8(14), 3781-3796 (2018-08-08)
Rationale: Tumor-associated fibroblasts (TAFs) play a critical role in the suppressive immune tumor microenvironment (TME), compromising the efficacy of immunotherapy. To overcome this therapeutic hurdle, we developed a nanoemulsion (NE) formulation to deliver fraxinellone (Frax), an anti-fibrotic medicine, to TAFs
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