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M8195

Sigma-Aldrich

Monoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse

~2 mg/mL, clone PH-MDMX-169, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-HDM4, Anti-HDMX, Anti-MDM4, Anti-MGC132766, Anti-MRP1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PH-MDMX-169, monoclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using total cell extract of HEK-293T cells co-transfected with human MDMX and a specific kinase

isotype

IgG2b

UniProt accession no.

O15151

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr99)

Gene Information

human ... MDM4(4194)

General description

MDMX is a nuclear protein and the gene encoding it is localized on human chromosome 1q32.1.
Monoclonal Anti-phospho-MDMX (pTyr99) (mouse IgG2b isotype) is derived from the hybridoma PH-MDMX-169 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse double minute 4 (MDM4) or MDMX comprises p53 binding domain, central acidic region, zinc and interesting new gene (RING) finger domain and is a phosphorylated protein. MDM4 gene is mapped to human chromosome 1q32.1.

Specificity

Monoclonal Anti-phospho-MDMX (pTyr99) recognizes human phosphorylated MDMX (pTyr99) (~ 80 kDa). The antibody does not recognize the non-phosphorylated MDMX, which is produced when MDMX is expressed with a kinase mutant.

Immunogen

synthetic phosphopeptide corresponding to amino acids 93-106 (pTyr99) of human MDMX.

Application

Monoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse may be used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunofluorescence
  • immunoblotting

Biochem/physiol Actions

MDMX associates with tumor suppressor p53 and inhibits its activity. It is upregulated in many tumors.
Studies began to unravel the regulation of murine double minute X (MDMX) in response to stress conditions, involving post-translational modifications and protein-protein interactions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaowen Wang et al.
Brain research, 1537, 260-266 (2013-09-03)
The product of the MDMX (or MDM4) gene is structurally related to the MDM2 oncoprotein and is also capable of interacting with the tumor suppressor protein p53. The MDM4 gene is overexpressed in several human tumors, while its product can
Qiong Yu et al.
Diagnostic pathology, 9, 71-71 (2014-03-29)
The p53 tumor suppressor gene is mutated or deleted in nearly half of human cancers. The murine double minute 2 (Mdm2) and Mdmx represent two important cellular regulators of p53. The aim of this study was to evaluate the abnormalities
F Mancini et al.
Current genomics, 10(1), 42-50 (2009-09-02)
MDM family proteins are crucial regulators of the oncosuppressor p53. Alterations of their gene status, mainly amplification events, have been frequently observed in human tumors.MDM4 is one of the two members of the MDM family. The human gene is located
Samanta Salvi et al.
International journal of molecular sciences, 15(7), 12458-12468 (2014-07-16)
Patients with non-muscle invasive bladder cancer (NMIBC) generally have a high risk of relapsing locally after primary tumor resection. The search for new predictive markers of local recurrence thus represents an important goal for the management of this disease. We
H Zhang et al.
Oncogene, 34(44), 5560-5569 (2015-02-24)
Inactivation of the retinoblastoma protein (RB) has a major role in the development of human malignancies. We have previously shown that MDM2, an ubiquitin E3 ligase and major negative regulator of p53, binds to and promotes proteasome-mediated degradation of RB.

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