Skip to Content
MilliporeSigma
All Photos(2)

Documents

M8318

Sigma-Aldrich

Anti-AtMPK3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Arabidopsis thaliana MAP Kinase 3

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 43 kDa

species reactivity

Arabidopsis thaliana

technique(s)

western blot: 1-2 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves
western blot: 2-4 μg/mL using extract (cytosolic fraction) of Nicotiana tabacum leaves

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Arabidopsis thaliana ... ATMPK3(823706)

General description

The protein kinase AtMPK3 is a component of the MAP kinase cascade.

Specificity

Anti-AtMPK3 recognizes AtMPK3.

Immunogen

Synthetic peptide corresponding to amino acids at the C-terminus of Arabidopsis thaliana MPK3. This sequence is specific to AtMPK3 (not found in the other 20 known AtMPK isoforms). It has limited homology (58% identity) to the AtMPK6 isoform and shares significant homology (67% identity, continuous epitope) with the Nicotiana tabacum ortholog WIPK.

Application

Anti-AtMPK3 antibody produced in rabbit has been used in immunoblotting analysis.

Biochem/physiol Actions

AtMPK3 is a component of the plant MAPK signaling cascade that functions downstream of the FLS2 receptor and regulates the innate response to bacterial and fungal infections. AtMPK3 is also involved in the phosphorylation of VIP1 in response to Agrobacterium infection and the subsequent transfer of the bacterial T-DNA into the nucleus. RNAi mediated inhibition of AtMPK6 causes prolonged activation of AtMPK3 . Anti-AtMPK3 antibody is specific for AtMPK3 (43 kDa). The product also reacts with Nicotiana tabacum and p56 in Papaver rhoeas . In immunoblotting, staining of AtMPK3 is specifically inhibited with AtMPK3 immunizing peptide (Arabidopsis thaliana, amino acids 359-370).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Still not finding the right product?  

Give our Product Selector Tool a try.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Armin Djamei et al.
Science (New York, N.Y.), 318(5849), 453-456 (2007-10-20)
Nuclear import of transfer DNA (T-DNA) is a central event in Agrobacterium transformation of plant cells and is thought to occur by the hijacking of certain host cell proteins. The T-DNA-associated virulence protein VirE2 mediates this process by binding to
Regulation of MAPKs by growth factors and receptor tyrosine kinases
Katz M, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1773(8) (2007)
Expression pattern and core region analysis of AtMPK3 promoter in response to environmental stresses
Gao F, et al.
Science China: Life Sciences, 53(11), 1315-1321 (2010)
Different protein kinase families are activated by osmotic stresses in Arabidopsis thaliana cell suspensions
Droillard MJ, et al.
Febs Letters, 527(1-3) (2002)
CBL-interacting protein kinase 6 negatively regulates immune response to Pseudomonas syringae in Arabidopsis
Sardar A, et al.
Journal of Experimental Botany, 68(13), 3573-3584 (2017)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service