MAK059
Formate Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
application(s)
cosmetics
environmental
food and beverages
detection method
colorimetric
storage temp.
−20°C
General description
Formate, the salt form of formic acid, is the simplest carboxylate anion. Formate is produced in the mitochondria as a result of the oxidation of one-carbon donors such as serine. Mitochondrially-derived formate is utilized for cytoplasmic one-carbon metabolism in the de novo synthesis of purines, thymdiylate, and the remethylation of homocysteine to methionine. Under normal physiologic conditions, formate is present at low levels in serum and blood but levels may be increased following acute methanol poisoning or environmental exposure to formaldehyde. Serum and urine formate levels were increased in vitamin B12-deficient rats and may be indicative of hyperhomocysteinemia due to defects in remethylation.
Application
Formate Assay kit has been used to determine the concentration of formate in samples.
Suitability
Suitable for serum, urine, media, cells, and tissues.
Principle
The Formate Assay Kit provides a simple, sensitive, and rapid means of quantifying formate in a variety of samples including serum, urine, media, cells, and tissues. Formate concentrations are determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the formate present.
Other Notes
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Danger
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Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
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WGK 3
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Simon G Lamarre et al.
American journal of physiology. Endocrinology and metabolism, 302(1), E61-E67 (2011-09-22)
Formate can differentiate between hyperhomocysteinemia due to impaired remethylation and impaired transsulfuration. Am J Physiol Endocrinol Metab 301: E000-E000, 2011. First published September 20, 2011; 10.1152/ajpendo.00345.2011.-We carried out a (1)H-NMR metabolomic analysis of sera from vitamin B(12)-deficient rats. In addition
Providing reducing power by microalgal photosynthesis: a novel perspective towards sustainable biocatalytic production of bulk chemicals exemplified for aliphatic amines
Lowe J, et al.
Scientific Reports, 8, 10436-10436 (2018)
Benjamin J Koestler et al.
mBio, 9(5) (2018-09-27)
The intracellular human pathogen Shigella flexneri invades the colon epithelium, replicates to high cell density within the host cell, and then spreads to adjacent epithelial cells. When S. flexneri gains access to the host cytosol, the bacteria metabolize host cytosolic
Jana Löwe et al.
Scientific reports, 8(1), 10436-10436 (2018-07-12)
A biotechnological process is reported, which enables an enzymatic reduction without the need for addition of an organic co-substrate for in situ-cofactor recycling. The process is based on merging the fields of enzymatic reductive amination with formate dehydrogenase-based in situ-cofactor
Ho Shing Chan et al.
Frontiers in microbiology, 12, 654033-654033 (2021-05-11)
Klebsiella pneumoniae with crude glycerol-utilizing and hydrogen (H2)-producing abilities was successfully isolated from return activated sludge from Shatin Sewage Treatment Works. The H2 production strategy used in this study was optimized with crude glycerol concentrations, and 1,020 μmol of H2
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