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MAK118

Sigma-Aldrich

Invertase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

β-fructofuranosidase Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric
fluorometric

storage temp.

−20°C

Related Categories

General description

Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.

Application

Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.

Principle

In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.

wgk_germany

WGK 2


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Identification and Functional Characterization of Sugarcane Invertase Inhibitor (ShINH1): A Potential Candidate for Reducing Pre-and Post-harvest Loss of Sucrose in Sugarcane.
Shivalingamurthy S G, et al.
Frontiers in Plant Science, 9 (2018)
Roua Gabriela Popescu et al.
Animals : an open access journal from MDPI, 10(5) (2020-05-14)
The purpose of this study was to examine the effects of dietary inclusion of two additives at the final concentration of 0.5% bilberry (E1) and 1% walnut (E2) leaves powder in the basal diet on digestive health of hens. A
Domenica Farci et al.
Journal of experimental botany, 67(11), 3303-3312 (2016-04-17)
Invertases are a widespread group of enzymes that catalyse the conversion of sucrose into fructose and glucose. Plants invertases and their substrates are essential factors that play an active role in primary metabolism and in cellular differentiation and by these
Khaled M Hazzouri et al.
Nature communications, 10(1), 4680-4680 (2019-10-17)
Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa. Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species. Here we report
Suresha G Shivalingamurthy et al.
Frontiers in plant science, 9, 598-598 (2018-05-19)
In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play

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