MAK464
Lactate Dehydrogenase Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
LDH Assay Kit, Lactate Dehydrogenase Activity Assay Kit
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.84
Recommended Products
Quality Level
application(s)
pharmaceutical
detection method
colorimetric
relevant disease(s)
cancer; hematological disorder
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage.
Features and Benefits
- Linear detection range 2-200 U/L
- Compatible with high-throughput handling systems.
- Simple procedure allows for reliable quantitation of LDH activity within 30 minutes.
Suitability
The kit is suitable for lactate dehydrogenase activity determination in serum, plasma, and other sources. It is also suitable for the characterization and quality control of LDH production, as well as for the screening and evaluation of LDH modulators in drug discovery.
Principle
The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
wgk_germany
WGK 2
Certificates of Analysis (COA)
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