MAK510
Chloride Assay Kit
sufficient for 250 colorimetric tests
Synonym(s):
Chloride Quantitation Kit
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About This Item
Quality Level
application(s)
cosmetics
environmental
food and beverages
pharmaceutical
detection method
colorimetric
relevant disease(s)
pulmonary disorders; endocrinological disorders; cardiovascular diseases; genitourinary disorders
shipped in
ambient
storage temp.
2-8°C
Related Categories
General description
Chloride is the major extracellular anion in human body fluids. Chloride plays a key role in maintaining proper water distribution, osmotic pressure and electrolyte balance. Low chloride concentrations may be found with prolonged vomiting, extensive burns, metabolic acidosis, Addisonia crisis and renal diseases. Elevated chloride concentrations are associated with dehydration, congestive heart failure, hyperventilation and urinary obstructions. Determination of chloride in sweat is useful in diagnosing cystic fibrosis.
Features and Benefits
- Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM)
- Compatible with high-throughput handling systems.
- The optimized formulation has greatly enhanced reagent and signal stability.
- Low interference in biological samples, no pretreatments are needed. Assays can be directly performed on raw biological samples in the presence of lipid, protein and minerals such as magnesium, iron and zinc.
Suitability
The kit is suitable for chloride determination in serum, plasma, urine, saliva, sweat, water, soil, food, and beverages, as well as for studying the effects of drugs on chloride metabolism.
Principle
The Chloride Assay Kit is designed to measure chloride directly in biological samples without any pretreatment. The improved Fried method utilizes mercuric 2,4,6-tripyridyl-s-triazine, which forms a colored complex specifically with chloride. The intensity of the color, measured at 610 nm, is directly proportional to the chloride concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2
wgk_germany
WGK 2
Certificates of Analysis (COA)
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