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MBD0014

Sigma-Aldrich

Microbial DNA standard from Pseudomonas Aeruginosa

Suitable for PCR, sequencing and NGS, ≥10 ng/μL

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About This Item

UNSPSC Code:
41105500
NACRES:
NA.24

Quality Level

200

form

liquid

concentration

≥10 ng/μL

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

suitability

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

shipped in

ambient

storage temp.

−20°C

Related Categories

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Pseudomonas Aeruginosa genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs. Pseudomonas aeruginosa is a gram negative aerobic to facultative anaerobe rod shaped bacteria which commonly inhabits soil and aqueous environments. In cases of a compromised immune system Pseudomonas aeruginosa can be an opportunistic pathogen. Its devastating effect is mostly known in Cystic Fibrosis (CF) patients. in addition, it often poses a major problem in burn wounds, chronic wounds, and in chronic obstructive pulmonary Disorder (COPD). P.aeruginosa forms biofilms on implanted biomaterials within hospital surface and water supplies. The international nosocomial infection control consortium reported that P. aeruginosa nosocomial infections have become a worldwide healthcare issue.




Read here how to use our standards to ensure data integrity for your microbiome research.

Application

The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physical form

Liquid -The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Montserrat Saladié et al.
Frontiers in microbiology, 11, 572504-572504 (2020-10-31)
In recent years the study of the commensal microbiota is driving a remarkable paradigm shift in our understanding of human physiology. However, intrinsic technical difficulties associated with investigating the Microbiomics of some body niches are hampering the development of new

Articles

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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