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Sigma-Aldrich

Monoclonal Anti-Na+/Ca2+ Exchanger antibody produced in mouse

clone C2C12

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About This Item

MDL number:
MFCD01865491
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody product type

primary antibodies

clone

C2C12, monoclonal

form

liquid

mol wt

antigen 120 kDa (mature exchanger)
antigen 160 kDa (non-reduced)
antigen 70 kDa (proteolytic fragment)

species reactivity

human, rat, canine, rabbit, guinea pig

technique(s)

immunohistochemistry (frozen sections): 1:200
immunoprecipitation (IP): suitable
western blot: 1:1,000

isotype

IgM

UniProt accession no.

P32418

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SLC8A1(6546)

General description

Na+/Ca2+ exchanger is also known as solute carrier family 8 member A1 (SLC8A1) and is present in the plasma membrane. It is predicted to have nine transmembrane segments and the gene encoding SLC8A1 has three promoters.

Specificity

The antibody reacts specifically with Na+/Ca2+ exchanger in kidney and cardiac tissues. By immunohistology, staining of the plasma membrane and intense staining of the cardiac T-tubular membrane is observed. The antibody is not recommended for immunoblotting of rat tissues.

Immunogen

dog cardiac Na+/Ca2+ exchanger. The epitope is in the region of amino acids 371-525, which is on the intracellular side of the plasma membrane.

Biochem/physiol Actions

Na+/Ca2+ exchanger (SLC8A1, solute carrier family 8 member A1) is involved in Ca2+ homeostasis. Depending on physiological conditions prevailing in the cell, it can either pump out Na+ or Ca2+.

Physical form

Solution in phosphate buffered saline, 1 mg/ml BSA containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Ping-Chun Li et al.
PloS one, 9(2), e85909-e85909 (2014-02-06)
Levosimendan, a known calcium sensitizer with positive inotropic and vasodilating properties, might also be cardioprotective during ischemia-reperfusion (I/R) insult. Its effects on calcium homeostasis and apoptosis in I/R injury remain unclear. Na(+)/Ca(2+) exchanger (NCX) is a critical mediator of calcium
Kathleen O'Connell et al.
Experimental gerontology, 43(10), 958-961 (2008-09-03)
In skeletal muscle, Ca(2+)-cycling through the sarcoplasm regulates the excitation-contraction-relaxation cycle. Since uncoupling between sarcolemmal excitation and fibre contraction may play a key role in the functional decline of aged muscle, this study has evaluated the expression levels of key
J S Frank et al.
The Journal of cell biology, 117(2), 337-345 (1992-04-01)
The present study reports on the location of the Na(+)-Ca2+ exchanger in cardiac sarcolemma with immunofluorescence and immunoelectron microscopy. Both polyclonal and monoclonal antibodies to the Na(+)-Ca2+ exchanger were used. The mAb was produced from a hybridoma cell line generated
Jia Chang et al.
The Journal of biological chemistry, 282(42), 30938-30948 (2007-08-28)
Mesenchymal stem cells (MSCs) are multipotent cells that can be differentiated into osteoblasts and provide an excellent cell source for bone regeneration and repair. Recently, the canonical Wnt/beta-catenin signaling pathway has been found to play a critical role in skeletal
Julian Vallejo et al.
PloS one, 11(3), e0150066-e0150066 (2016-03-10)
There is growing evidence that severe decline of skeletal muscle mass and function with age may be mitigated by exercise and dietary supplementation with protein and amino acid ingredient technologies. The purposes of this study were to examine the effects
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