N6288
Anti-Nucleolin−Atto 488 antibody produced in rabbit
1.5-3.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-NCL, Protein C23
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
Atto 488 conjugate
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
human, mouse, rat
storage condition
protect from light
concentration
1.5-3.0 mg/mL
technique(s)
direct immunofluorescence: 0.5-1.0 μg/mL using human HeLa, rat NRK and mouse 3T3 cells
fluorescence
λex 500 nm; λem 522 nm in PBS
λex 500 nm; λem 522 nm
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NCL(4691)
mouse ... Ncl(17975)
rat ... Ncl(25135)
General description
Nucleolin (NCL) is an anti-apoptotic phosphoprotein. It is highly expressed in the nucleolus as well as other sub-cellular organelles.
Find more information here
Find more information here
Nucleolin is a multi-domain phosphoprotein mapped to human chromosome 2q37. It comprises three domains and a bipartite nuclear localization signal sequence. Nucleolin domains include the highly acidic N-terminal region, a RNA-binding central domain and a glycine, arginine and phenylalanine residues rich C-terminal region.
Specificity
Anti-Nucleolin-Atto 488 recognizes human, rat, and mouse nucleolin.
Immunogen
synthetic peptide corresponding to amino acid residues 2-17 of human nucleolin conjugated to KLH. The corresponding sequence differs by 4 amino acids in rat and mouse nucleolin, and by one amino acid in the rat homolog protein NRP (nucleolin related protein).
Application
Anti-Nucleolin−Atto 488 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence.
Biochem/physiol Actions
Nucleolin (NCL) interacts with proteins participating in microtubule nucleation. It takes part in the transcription of polymerase I, formation of ribosomes and duplication of centrosomes. NCL is also a transcription regulator. Studies have shown that it is highly expressed in many tumors.
Nucleolin N- and C-terminal domains are involved in protein-protein interaction, whereas the central domain is involved in specific interactions with nucleic acids. Nucleolin exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin′s multiple activities may be regulated by proteolysis and methylation. It also interacts with telomerase andthis interaction is critical for the nucleolar localization of telomerase. Nucleolin might function as a cell surface receptor for lipoproteins, viruses, extracellular matrix, growth factors and other molecules.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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The roles of nucleolin subcellular localization in cancer.
Berger CM
Biochimie, 113, 78-85 (2015)
Jian-Yu Xu et al.
Asian Pacific journal of cancer prevention : APJCP, 16(8), 3301-3306 (2015-04-30)
Nucleolin (C23) is an important anti-apoptotic protein that is ubiquitously expressed in exponentially growing eukaryotic cells. In order to understand the impact of C23 in radiation therapy, we attempted to investigate the relationship of C23 expression with the radiosensitivity of
Induced expression of nucleolin phosphorylation-deficient mutant confers dominant-negative effect on cell proliferation.
Xiao S
PLoS ONE, 9(10), e109858-e109858 (2014)
Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Laura Fabre et al.
Biomacromolecules, 23(6), 2302-2314 (2022-05-14)
Nucleolin is a multifunctional protein involved in essential biological processes. To precisely localize it and unravel its different roles in cells, fluorescence imaging is a powerful tool, especially super-resolution techniques. Here, we developed polymer-aptamer probes, both small and bright, adapted
Articles
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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