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N6537

Sigma-Aldrich

Anti-NPTII antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Neomycin Phosphotransferase II

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About This Item

MDL number:
MFCD01633903
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 30 kDa

species reactivity

plant

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

western blot: 2-4 μg/mL using extract (cytosolic fraction) from the leaves of transgenic Nicotiana tabacum expressing E. coli NPTII

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from the E. coli transposon Tn5.

Specificity

The antibody recognizes bacterial NPTII expressed in transgenic tobacco plants.

Immunogen

synthetic peptide corresponding to amino acids 133-150 located at the mid-region of E. coli NPTII, conjugated to KLH.

Application

Anti-NPTII antibody has been used in immunoblotting.

Biochem/physiol Actions

The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure
Yenofsky R L, et al.
Proceedings of the National Academy of Sciences of the USA, 87(9), 3435-3439 (1990)
Purification and characterization of microbially expressed neomycin phosphotransferase II (NPTII) protein and its equivalence to the plant expressed protein
Fuchs R L, et al.
Biotechnology, 11(12), 1537-1537 (1993)

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