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N8002

Sigma-Aldrich

Nuclear Fast Red

Synonym(s):

Calcium Red, 4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt

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About This Item

Empirical Formula (Hill Notation):
C14H8NNaO7S
CAS Number:
6409-77-4
Molecular Weight:
357.27
Colour Index Number:
60760
EC Number:
229-088-0
MDL number:
MFCD00078493
UNSPSC Code:
12171500
PubChem Substance ID:
24897846
NACRES:
NA.47

form

powder

Quality Level

200

solubility

water: soluble 4 mg/mL, dark red to very dark red and red purple

suitability

suitable for determination of calcium

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Nc1c(O)c(c(O)c2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O

InChI

1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1

InChI key

IFSXZLJQEKGQAF-UHFFFAOYSA-M

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Application

Nuclear Fast Red is suitable for the determination of calcium. It is suitable for use in prussian blue staining in neural progenitor cells. It may be employed as highly sensitive "off/on" fluorescent probe for the determination of guanine. Nuclear fast red (2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt) may be used as selective complexing agent for the simultaneous determination of copper and bismuth by adsorptive stripping voltammetry.
Used as a biological stain

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A micromethod for the estimation of serum calcium.
S BAAR
Clinica chimica acta; international journal of clinical chemistry, 2(6), 567-575 (1957-12-01)
Yi Cui et al.
Talanta, 130, 536-541 (2014-08-28)
A novel fluorescence method for the determination of guanine was developed based on the fluorescence enhancement of Cu(2+)-nuclear fast red complex in the presence of guanine in Tris-HCl buffer. The complex of Cu(2+) with nuclear fast red resulted in a
Preparation of Polypyrrole/Nuclear Fast Red Films on Gold Electrode and Its Application on the Electrocatalytic Determination of Methyl-dopa and Ascorbic Acid.
Gholivand MB and Amiri M.
Electroanalysis, 21(22), 2461-2467 (2009)
M B Gholivand et al.
Analytica chimica acta, 571(1), 99-104 (2007-08-29)
A sensitive and selective method for the simultaneous determination of copper and bismuth by adsorptive stripping was developed using nuclear fast red (2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt) as selective complexing agent onto hanging mercury drop electrode. In a single scan
Mohammad-Bagher Gholivand et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 115, 516-527 (2013-07-23)
For the first time, interaction of nuclear fast red (NFR) with human serum albumin (HSA) was studied by experimental and computational approaches. Firstly, experimental measurements including fluorescence spectroscopy (F), UVvis spectrophotometry (UVvis), cyclic voltammetry (CV), differential pulse voltammetry (DPV) and
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