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O8389

Sigma-Aldrich

Anti-Oct 3/4 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-OTF3/OTF4, Anti-Octamer-binding transcription factor 3/4, Anti-POU class 5 homeobox 1, Anti-POU5F1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human, rat

concentration

~1 mg/mL

technique(s)

immunohistochemistry: 1:100-1:250
western blot: 1:500-1:1,000

UniProt accession no.

Q01860

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)

General description

POU domain class 5 transcription factor 1 (POU5F1) is also termed as OCT4. It is a transcription factor. OCT4 codes for three isoforms, such as OCT4A, OCT4B and OCT4B1. These isoforms are produced by alternative splicing. The OCT4 gene consists of 7 exons. This gene is located on human chromosome 6. OCT4A and OCT4B are located in the nucleus and cytoplasm respectively.

Immunogen

synthetic peptide corresponding to an internal sequence of human Oct 3/4. This sequence is 84% identical in mouse and 94% identical in rat.

Application

Anti-Oct 3/4 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence staining.

Biochem/physiol Actions

Oct 3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct 3/4 stimulates loss of pluripotency and dedifferentiation to trophectoderm. POU domain class 5 transcription factor 1 (POU5F1) serves as a master regulator of pluripotency in embryonic stem cells. It is one of the reprogramming factors, that is highly essential for generating induced pluripotent stem cells.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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OCT4B Isoform Promotes Anchorage-Independent Growth of Glioblastoma Cells
Choi SH, et al.
Molecules and cells, 42(2), 135-135 (2019)
Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
Niwa H, et al.
Nature Genetics, 24(4), 372-376 (2000)
Optimization of Serum-Free Culture Conditions for Propagation of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells
Sharma A, et al.
Cellular Reprogramming, 21(1) (2019)
Michalina Alicka et al.
Stem cell research & therapy, 11(1), 4-4 (2020-01-05)
Progressive loss of cell functionality caused by an age-related impairment in cell metabolism concerns not only mature specialized cells but also its progenitors, which significantly reduces their regenerative potential. Adipose-derived stem cells (ASCs) are most commonly used in veterinary medicine
Luca Ampollini et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 46(6), e103-e112 (2014-10-15)
The aim of the present study was to characterize the biological properties and in vivo tumourigenic potential of mesenchymal cells (MCs) obtained from non-small-cell lung cancer (NSCLC) samples. NSCLC samples (53 adenocarcinomas and 24 squamous-cell carcinomas) surgically removed from 46

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