P1903
Pyruvate Kinase from Bacillus stearothermophilus
Type VIII, lyophilized powder, 100-300 units/mg protein
Synonym(s):
ATP:pyruvate 2-O-phosphotransferase, PK
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About This Item
Recommended Products
biological source
Bacillus sp. (B. stearothermophilus)
Quality Level
type
Type VIII
form
lyophilized powder
specific activity
100-300 units/mg protein
storage temp.
2-8°C
Application
Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.
Biochem/physiol Actions
Pyruvate Kinase from Bacillus stearothermophilus is activated by AMP and ribose 5-phosphate.
Unit Definition
One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.2 at 30 °C.
Physical form
Lyophilized powder containing Tris buffer salts, pH 8.5
Analysis Note
Protein determined by biuret
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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E W Walters et al.
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Molecular cloning and nucleotide sequence of the gene for pyruvate kinase of Bacillus stearothermophilus and the production of the enzyme in Escherichia coli. Evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon
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FEBS Journal, 211, 851-859 (1993)
Crystallization and preliminary X-ray analysis of pyruvate kinase from Bacillus stearothermophilus
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Acta Crystallographica Section B, Structural Crystallography and Crystal Chemistry, F61, 759-761 (2005)
Mutagenesis of the active site lysine 221 of the pyruvate kinase from Bacillus stearothermophilus
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The Journal of Biological Chemistry, 137, 141-145 (2005)
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