Skip to Content
MilliporeSigma
All Photos(1)

Documents

P7123

Sigma-Aldrich

Protein Kinase Cν (PKCν) Active human

recombinant, expressed in E. coli, ≥75% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

PKD3, Protein Kinase C nu

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

assay

≥75% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

protein apparent mol wt ~142 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKD3(23683)

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide (KRREILSRRPSYR) per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Biochem/physiol Actions

PKCν, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCν is an important physiologic target of the B-cell receptor (BCR) and exhibits activation upon BCR engagement.

Physical form

Solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service