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P7457

Sigma-Aldrich

Carboxy-terminal FLAG-BAP™ Fusion Protein

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About This Item

MDL number:
MFCD00130921
UNSPSC Code:
12352202
NACRES:
NA.32

form

liquid

Quality Level

200

mol wt

~49 kDa

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).

Application

Carboxy-terminal FLAG-BAP™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).
Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.

Other Notes

Control protein

Physical form

Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2

Preparation Note

Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

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Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels
Domanski M, et al.
Biotechniques, 1-1 (2012)
Microbial alkaline phosphatases in bioprocessing
Nalini P, et al.
International Journal of Current Microbiology and Applied Sciences, 4(3), 384-396 (2015)
Maddalena de Virgilio et al.
Journal of experimental botany, 59(10), 2815-2829 (2008-06-10)
Protein bodies (PB) are stable polymers naturally formed by certain seed storage proteins within the endoplasmic reticulum (ER). The human immunodeficiency virus negative factor (Nef) protein, a potential antigen for the development of an anti-viral vaccine, is highly unstable when
Patricia L Pelczar et al.
Journal of bacteriology, 190(16), 5635-5641 (2008-06-17)
GerD of Bacillus subtilis is a protein essential for normal spore germination with either L-alanine or a mixture of L-asparagine, D-glucose, D-fructose, and potassium ions. GerD's amino acid sequence suggests that it may be a lipoprotein, indicating a likely location
Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells
Chubet RG and Brizzard BL
Biotechniques, 20(1), 136-141 (1996)
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Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

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Protein Purification

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Protein Expression

Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.

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