QR0200
Quantitative RT-PCR ReadyMix™
One step RT-qPCR for probe-based methods, MMLV & hot-start Taq
Synonym(s):
1-step RT-qPCR mix, Quantitative real-time PCR master mix
About This Item
Recommended Products
Quality Level
usage
sufficient for 250 reactions
feature
dNTPs included
hotstart
technique(s)
RT-qPCR: suitable
color
colorless
input
purified RNA
compatibility
ABI 5700
ABI 7000
ABI 7300
ABI 7500 Fast
ABI 7500
ABI 7700
ABI 7900 HT
ABI 7900 HT Fast
ABI 7900
ABI StepOne
ABI StepOnePlus
ABI ViiA 7
Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex2 s
Eppendorf® Mastercycler ep realplex
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000
detection method
probe-based
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Application
Features and Benefits
- The master mix allows consistency and reproducibility from one reaction to the next
- Reduced risk of contamination from multiple pipetting steps
- Reduced set-up time as compared to manual or wax Hot Start methods
- JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
- Broad instrument compatibility
- Includes a separate ROX reference dye vial for reaction normalization
Packaging
Legal Information
Kit Components Only
- Probe Based qRT-PCR ReadyMix 2 X
- Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U
Kit Components Also Available Separately
related product
signalword
Warning
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Related Content
RT-qPCR, or quantitative reverse transcription PCR, combines the effects of reverse transcription and quantitative PCR or real-time PCR to amplify and detect specific targets. RT-qPCR has a variety of applications including quantifying gene expression levels, validating RNA interference (RNAi), and detecting pathogens such as viruses.
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