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R106-05N

Sigma-Aldrich

Rat Dermal Fibroblasts: RDF, neonatal

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

rat dermis

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

morphology

Fibroblast

technique(s)

cell culture | mammalian: suitable

storage temp.

−196°C

Related Categories

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.


RDF from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration (Nomoto, 2012), in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing (Muraoka, 2013), and as a control in engineered cardiac tissues (Serrao, 2012).

Cell Line Origin

Skin

Application

Injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response.

Components

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Preparation Note

  • 1st passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 8 population doublings

Subculture Routine

Please refer to the RDF Culture Protocol.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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