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R7638

Sigma-Aldrich

RPMI-1640 Medium

Dutch Modification, with sodium bicarbonate and HEPES, without ʟ-glutamine, sterile liquid, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.75

product name

RPMI-1640 Medium, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used

  • to obtain spleen and lymph nodes from mice
  • for the resuspension of Staphylococcus aureus strains in the phagocytosis assay
  • in the isolation of hepatitis B surface antigen-specific B-cell clones

Reconstitution

Supplement with 0.3 g/L L-glutamine.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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