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R7904

Sigma-Aldrich

Anti-Rab5 antibody, Mouse monoclonal

clone Rab5-65, purified from hybridoma cell culture

Synonym(s):

Anti-RAS-associated Protein RAB5A

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About This Item

MDL number:
MFCD01322589
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Rab5-65, monoclonal

form

buffered aqueous solution

mol wt

~24 kDa

species reactivity

human, cat, bovine, mouse, rat

concentration

~2 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HeLa cells or NIH-3T3 or NRK
indirect ELISA: suitable
microarray: suitable
western blot: suitable

isotype

IgG3

UniProt accession no.

P20339

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)

General description

Anti-Rab5 antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the hybridoma Rab5-65 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to C-terminal region of human Rab5, conjugated to KLH. Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane.
Rab5 (Ras-related protein Rab-5A) is a small GTPase , that has three isoforms - Rab5A, Rab5B and Rab5C. It is upregulated in several secretory cells. It is located on human chromosome 3p24.

Immunogen

synthetic peptide corresponding to the C- terminal region of human Rab5, conjugated to KLH.

Application

Anti-Rab5 antibody, Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • co-immunoprecipitation assay
  • western blotting
  • immunofluorescence

Biochem/physiol Actions

Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and early endosome antigen 1(EEA1).
Rab5 (Ras-related protein Rab-5A) controls endosomal trafficking. Overexpression of Rab5A leads to metastasis and poor prediction in colorectal cancer patients. It modulates common Rab5 effectors to control the secretory pathways. It controls the homotypic integration between early endosomes and also the integration between endocytic vesicles and early endosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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The Autophagic Process Occurs in Human Bone Metastasis and Implicates Molecular Mechanisms Differently Affected by Rab5a in the Early and Late Stages
Maroni P, et al.
International Journal of Molecular Sciences, 17(4) (2016)
Probing the druggability of membrane-bound Rab5 by molecular dynamics simulations
Edler E and Stein M
Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 434-443 (2017)
Pamela A Godoy et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 142, 111968-111968 (2021-08-04)
Amyloid beta peptide (Aβ) is tightly associated with the physiopathology of Alzheimer's Disease (AD) as one of the most important factors in the evolution of the pathology. In this context, we previously reported that Aβ increases the expression of ionotropic
Prolonged morphine treatment alters delta opioid receptor post-internalization trafficking
Ong EW, et al.
British Journal of Pharmacology, 172(2), 615-629 (2015)
Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7
Zhao P, et al.
Biochimica et biophysica acta. General subjects, 1863(2), 502-510 (2019)
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