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R9010

Sigma-Aldrich

Monoclonal Anti-Rat Kappa Light Chains (1a + 1b) antibody produced in mouse

clone RT-39, ascites fluid

Synonym(s):

Monoclonal Anti-Rat Kappa Light Chains (1a + 1b)

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About This Item

MDL number:
MFCD00164734
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

RT-39, monoclonal

mol wt

antigen 25 kDa

contains

15 mM sodium azide

technique(s)

capture ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: 1:2,000
western blot: suitable using denatured and reduced normal serum or myeloma protein

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Monoclonal anti-Rat Light Chain (1a+1b) (mouse IgG1 isotype) is derived from the RT-39 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG. An immunoglobulin (Ig) has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. Rat immunoglobulins have either κ or λ light chains. Greater than 90% of normal rat immunoglobulins and myelomas contain light chains.

Specificity

The antibody reacts with an epitope on the rat κ light chain, which is shared by both the 1a and 1b allotypes in various rat immunoglobulin classes and subclasses. No cross-reactivity is observed with rat λ light chains or with IgG from human, mouse, bovine, rabbit, goat, sheep, horse, dog, chicken, pig or cat. The product shows weak cross-reaction with guinea pig IgG.

Immunogen

Purified rat IgG

Application

Monoclonal Anti-Rat κ Light Chains (1a + 1b) antibody has been used in capture enzyme linked immunosorbent assay (ELISA), immunoblotting, dot blot and immunocytochemistry.
Monoclonal Anti-Rat Kappa Light Chains (1a + 1b) antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • dot blot
  • immunocytochemistry

Biochem/physiol Actions

Immunoglobulin G (IgG) mainly helps in immune defense. It participates in hypersensitivity type II and type III reactions. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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flash_point_f

Not applicable

flash_point_c

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The Laboratory Rat (1998)
The Immunoglobulins: Structure and Function (1998)
Molecular Genetics of Immunoglobulin, 17 (1987)
Scott G Filler et al.
The journal of investigative dermatology. Symposium proceedings, 12(1), 52-56 (2007-05-16)
Immunologic limitations make it difficult to study the pharmacokinetic effects of human tumor necrosis factor (TNF) blockers in murine models. To counter this, we have studied the pharmacokinetics in mice of two murine analogs of human TNF blockers, a murine
Pharmacokinetics of Murine p75-Fc Fusion Protein and MP6-XT22 Anti-Murine TNF-α mAb in Mice
Filler, S., G., et al.
The Journal of Investigative Dermatology, 12, 52-56 (2007)
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