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RAB0429

Sigma-Aldrich

Human PAI-1 ELISA Kit

for serum, plasma, cell culture supernatant.

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 35 pg/mL
standard curve range: 0.102-25 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SERPINE1(5054)

Related Categories

General description

The Human PAI-1 (plasminogen activator inhibitor-1) ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PAI-1 in serum, plasma, cell culture supernatants. PAI-1 is 45kDa serine proteinase inhibitor. It inhibits plasmogen activator and affects the fibrinolytic process, as a result of which it leads to thrombosis. Reduced fibrinolysis is observed in ischemic heart disease. While elevated PAI-1 levels are noticed in myocardial infarction. PAI-1 is associated with various physiological processes including wound healing, sepsis, insulin resistance, obesity, atherosclerosis, tumor angiogenesis, bone remodeling, asthma, rheumatoid arthritis, glomerulonephritis and chronic stress.

Immunogen

Recombinant Human PAI-I

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

wgk_germany

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Brain Edema XI: Proceedings of the 11th International Symposium, 277-277 (1999)
Pleiotropic functions of plasminogen activator inhibitor-1
Lijnen, HR
Journal of Thrombosis and Haemostasis, 3(1), 35-45 (2005)
María Paniagua-Sancho et al.
American journal of nephrology, 52(9), 714-724 (2021-09-15)
Acute kidney injury (AKI) is a threatening, multiaetiological syndrome encompassing a variety of forms and damage patterns. AKI lacks sufficiently specific diagnostic tools to evaluate the distinct combination of pathophysiological events underlying each case, which limits personalized and optimized handling.
Gui Yang et al.
The Journal of allergy and clinical immunology, 133(6), 1702-1708 (2014-04-05)
The functions of regulatory T (Treg) cells are important in immunity, and the regulatory mechanisms of Treg cell activities are not fully understood yet. We sought to investigate the role of insulin-like growth factor (IGF) 2 in the upregulation of

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