Skip to Content
MilliporeSigma
All Photos(1)

Documents

S200HR

Sigma-Aldrich

Sephacryl®

200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

Synonym(s):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
65546-95-4
MDL number:
MFCD00165824
UNSPSC Code:
12352202
NACRES:
NA.56

Quality Level

200

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

1-80 kDa MW range (dextrans)
5-250 kDa MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S200HR-750ML′s updated product number is GE17-0584-01

Application

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 6

1 of 6

Sephacryl® S-300 HR Cytiva 17-0599-10, pack of 150 mL

GE17-0599-10

Sephacryl® S-300 HR

Microspin™ S-200 HR Cytiva 27-5120-01, pack of 50 columns

GE27-5120-01

Microspin™ S-200 HR

Superdex® Superdex®200 Prep Grade

Sigma-Aldrich

S6782

Superdex®

Sepharose™ 6B 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)

Sigma-Aldrich

6B100

Sepharose™ 6B

Sephacryl® S-100 HR Cytiva 17-0612-10, pack of 150 mL

GE17-0612-10

Sephacryl® S-100 HR

Sephadex® G-100 Medium

Sigma-Aldrich

G100120

Sephadex® G-100

Zhili Xu et al.
Methods in enzymology, 468, 147-165 (2009-01-01)
Chemical probing is widely used as a rapid approach for assessing RNA structure, folding, and function. In this chapter, we outline procedures for handling and using chemicals commonly used to probe nucleic acids. Detailed experimental conditions and design for footprinting
Sudarshan Rajagopal et al.
Photochemistry and photobiology, 80(3), 542-547 (2004-09-30)
The Escherichia coli protein YcgF contains a photosensory flavin adenine dinucleotide (FAD)-binding BLUF domain covalently linked to an EAL domain, which is predicted to have cyclic-di-guanosine monophosphate (GMP) phosphodiesterase activity. We have cloned, overexpressed and purified this protein, which we
Andor J Kiss et al.
The Journal of experimental biology, 207(Pt 26), 4633-4649 (2004-12-08)
The eye lenses of the Antarctic nototheniid fishes that inhabit the perennially freezing Antarctic seawater are transparent at -2 degrees C, whereas the cold-sensitive mammalian and tropical fish lenses display cold-induced cataract at 20 degrees C and 7 degrees C
D J Flavell et al.
British journal of cancer, 84(4), 571-578 (2001-02-24)
Severe combined immunodeficient (SCID) mice injected i.v. with the human T-ALL cell line CCRF CEM (SCID-CEM mice) develop within 50 days life-threatening multi-organ growth of leukaemia cells. The development of leukaemia in SCID-CEM mice treated with three 10 microg i.v.
M Hamilton et al.
The Journal of biological chemistry, 276(31), 29079-29090 (2001-05-19)
Phorbol ester stimulation of the MAPK cascade is believed to be mediated through the protein kinase C (PKC)-dependent activation of Raf-1. Although several studies suggest that phorbol ester stimulation of MAPK is insensitive to dominant-negative Ras, a requirement for Ras
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service