Skip to Content
MilliporeSigma
All Photos(3)

Documents

S4072

Sigma-Aldrich

Anti-Sirt3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-SIR2L3, Anti-Sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

MDL number:
MFCD07784765
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

human

technique(s)

indirect immunofluorescence: suitable
western blot: 2.5-5 μg/mL using human HepG2 cells

UniProt accession no.

Q9NTG7

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SIRT3(23410)

Related Categories

General description

Sirtuin 3 (Sirt3) is an NAD-dependent deacetylase localized to the mitochondrial matrix. SIRT3 encodes a 44 kDa full length form and a N-terminal 142 amino acids truncated form. It is mapped to human chromosome 11p15.5.

Immunogen

synthetic peptide corresponding to amino acids 384-399 of human SIRT3, conjugated to KLH via an N-terminal cysteine residue.

Application

Anti-Sirt3 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Sirtuin 3 (Sirt3) is mainly a nuclear protein that transfers to the mitochondria during cellular stress. In humans, Sirt3 deacetylates and activates the mitochondrial enzyme acetyl-CoA synthetase 1 (AceCS2). Sirt3 is implicated in regulating mitochondrial function and thermogenesis in brown adipocytes. Its levels have been shown to correlate with extended life span. Increased levels of Sirt3 were associated with node positive breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

SIRT2 promotes the migration and invasion of gastric cancer through RAS/ERK/JNK/MMP-9 pathway by increasing PEPCK1-related metabolism
Li Y, et al.
Neoplasia, 20(7), 745-756 (2018)
SirT3 is a nuclear NAD+-dependent histone deacetylase that translocates to the mitochondria upon cellular stress
Scher MB, et al.
Genes & Development, 21(8), 920-928 (2007)
Characterization of a bidirectional promoter shared between two human genes related to aging: SIRT3 and PSMD13
Bellizzi D, et al.
Genomics, 89(1), 143-150 (2007)
Jing Zhang et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(12), 4418-4431 (2014-03-22)
Modern society enables a shortening of sleep times, yet long-term consequences of extended wakefulness on the brain are largely unknown. Essential for optimal alertness, locus ceruleus neurons (LCns) are metabolically active neurons that fire at increased rates across sustained wakefulness.
SIRT3: oncogene and tumor suppressor in cancer
Torrens-Mas M, et al.
Cancers, 9(7), 90-90 (2017)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service