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S6797

Sigma-Aldrich

Monoclonal Anti-S100A2 antibody produced in mouse

clone SH-L1, ascites fluid

Synonym(s):

Anti-S100L

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About This Item

MDL number:
MFCD00677383
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SH-L1, monoclonal

contains

15 mM sodium azide

species reactivity

lizard, goat, rat, bovine, human, feline, frog, canine, pig

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable using native preparations
indirect immunofluorescence: 1:1,000 using cultured Madin-Darby bovine kidney (MDBK) cells
western blot: 1:1,000

isotype

IgG1

UniProt accession no.

P29034

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... S100A2(6273)

General description

Monoclonal Anti-S100A2 (S100L) (mouse IgG1 isotype) is derived from the SH-L1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. S-1002 is a set of small, thermolabile, highly acidic 10-20 kDa dimer proteins that are widely distributed in different tissues. The S-100 family consists of at least 10 members with a cell-type-specific expression pattern. It is a calcium-modulated protein. S100A2 is also called S100L. S100A2 has been shown to possess 43-47% homology with S-100α, S-100β and Calcyclin. It is expressed at high levels in kidney and lung, intermediate levels in muscle and low levels in brain and intestine.

Specificity

The antibody recognizes an epitope located on S100A2 (S100L is the old terminology) in a Ca2+ ion-dependent manner. The product does not react with other members of the EF-hand family such as calmodulin, parvalbumin, intestinal calcium-binding protein, S100A6 (calcyclin), caltropin, the α chain of S-100 (i.e. in S-100a and S-100ao), or the β chain (i.e. in S-100a and S-100b).

Immunogen

Ca2+-binding proteins from pig stomach tissue

Application

Monoclonal Anti-S100A2 antibody has been used in enzyme linked immunosorbent assay (ELISA), immunoblotting, immunoprecipitation, immunohistology, western blotting.

Biochem/physiol Actions

S-100 binds to calcium and zinc ions reversibly at physiologic pH and ionic strength, followed by a conformational change in the molecule. S-100 is a cell-growth regulator, increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes. High levels of S100A2 are found also in the MDBK (Madin-Darby bovine kidney) cell line and in human glioma.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Differential expression patterns of S100A2, S100A4 and S100A6 during progression of human malignant melanoma
Maelandsmo G M, et al.
International Journal of Cancer. Journal International Du Cancer, 74(4), 464-469 (1997)
Joshua P Klopper et al.
PPAR research, 2010, 729876-729876 (2009-10-28)
Nuclear hormone receptors, including RXR and PPARgamma, represent novel therapeutic targets in melanoma. We have previously shown that the DRO subline of the amelanotic melanoma A375 responds to rexinoid and thiazolidinedione (TZD) treatment in vitro and in vivo. We performed
Jochen Jaeger et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 13(3), 806-815 (2007-02-10)
To better understand the molecular mechanisms of malignant melanoma progression and metastasis, gene expression profiling was done of primary melanomas and melanoma metastases. Tumor cell-specific gene expression in 19 primary melanomas and 22 melanoma metastases was analyzed using oligonucleotide microarrays
Combination PPAR and RXR Agonist Treatment in Melanoma Cells: Functional Importance of S100A2
Klopper J P, et al.
PPAR Research, 2010(4), 464-469 (2010)
M Gimona et al.
Journal of cell science, 110 ( Pt 5), 611-621 (1997-03-01)
Zero-length chemical crosslinking with 1-ethyl-3-[3-(dimethyl amino)propyl]carbodiimide (EDC) indicated an association of the Ca2+-binding protein S100A2 with tropomyosin (TM) in vitro. The mobility of the crosslinked product on SDS-PAGE gels indicated the formation of a 1:1 complex between S100A2 and TM

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