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S7945

Sigma-Aldrich

Monoclonal Anti-Siah2 antibody produced in mouse

clone Siah2-369, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Seven in Absentia Homolog

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About This Item

MDL number:
MFCD08705601
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Siah2-369, monoclonal

form

buffered aqueous solution

mol wt

antigen ~37 kDa

species reactivity

monkey, hamster, bovine, mouse, human, canine

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using total cell extract of HeLa cells

isotype

IgG1

UniProt accession no.

O43255

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SIAH2(6478)
mouse ... Siah2(20439)

Related Categories

General description

Monoclonal Anti-Siah2 (mouse IgG1 isotype) is derived from the hybridoma Siah2-369 produced by the fusion of mouse myeloma cells (NS1 cell) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 2-17 of human Siah2, conjugated to KLH. Seven in absentia homologue (Siah) is encoded by the gene mapped to human chromosome 3q25.1. Three different Siah genes exist in mice, Siah1a, Siah1b, and Siah2, while humans have only two genes, Siah1and Siah2.

Immunogen

synthetic peptide corresponding to amino acids 2-17 of human Siah2, conjugated to KLH.

Application

Monoclonal Anti-Siah2 antibody produced in mouse can be used in enzyme linked immunoassay (ELISA) and immunocytochemistry. It has also been used in immunohistochemistry and immunoblotting.

Biochem/physiol Actions

Seven in absentia homologue (Siah) plays a role in the regulation of key proteins in the immune system such as TNF receptor associated factors (TRAF), vav guanine nucleotide exchange factor 1 (Vav1), and oct-binding factor 1 (OBF-1). Siah2 is important for the regulation of the PHD enzymes (prolylhydroxylases) that are responsible for the prolyhydroxylation of HIFα protein under hypoxia conditions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Prolyl hydroxylase domain enzymes: important regulators of cancer metabolism
Yang M, et al.
Hypoxia (Auckland, N.Z.), 2(24), 127-127 (2014)
Wnt5a induces and maintains prostate cancer cells dormancy in bone
Ren D, et al.
The Journal of Experimental Medicine, 216(2), 428-449 (2019)
A genome-wide association study identifies a genetic variant in the SIAH2 locus associated with hormonal receptor-positive breast cancer in Japanese
Elgazzar S, et al.
Journal of Human Genetics, 57(12), 766-766 (2012)
Generation and analysis of Siah2 mutant mice
Frew IJ, et al.
Molecular and Cellular Biology, 23(24), 9150-9161 (2003)
Hypoxia regulates Hippo signaling through the SIAH2 ubiquitin E3 ligase
Ma B, et al.
Nature Cell Biology, 17(1), 95-95 (2015)
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