Skip to Content
MilliporeSigma
All Photos(1)

Documents

SAB2500450

Sigma-Aldrich

Anti-GAPDH, C-Terminal antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

G3PD, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, HEL-S-162eP, HGNC:4141, MGC88685, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

goat

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

canine, pig

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

UniProt accession no.

P04406

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GAPDH(2597)

Related Categories

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein and is encoded by the gene mapped to human chromosome 12p13. It is basically called as a glycolytic enzyme.

Immunogen

Peptide with sequence C-HQVVSSDFNSDT from the C Terminus of the protein sequence according to NP_002037.2.

Application

Recommended concentration:
Western Blot 0.01-0.5ug/uL
Immunohistochemistry 5 ug/mL
Immunofluorescence 5 ug/mL
Optimal dilutions/concentrations should be determined by the end user.
Anti-GAPDH, C-Terminal antibody produced in goat has been used in single cell western blot analysis.

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays an important role in DNA repair. It is essential for the sixth step of glycolysis. It participates in transcriptional and posttranscriptional gene regulation. It also controls intracellular membrane trafficking and cell death. Accumulation of GAPDH results in mitochondrial dysfunction. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

recommended

Product No.
Description
Pricing

wgk_germany

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Anti-GAPDH antibody, Mouse monoclonal clone GAPDH-71.1, purified from hybridoma cell culture

Sigma-Aldrich

G8795

Anti-GAPDH antibody, Mouse monoclonal

Increased copy number of the TERT and TERC telomerase subunit genes in cancer cells.
Cao Y, et al.
Cancer Science, 99(6), 1092-1099 (2008)
High-selectivity cytology via lab-on-a-disc western blotting of individual cells.
Kim J J, et al.
Lab on a chip, 17(5), 855-863 (2017)
Elly Sinkala et al.
Nature communications, 8, 14622-14622 (2017-03-24)
Circulating tumour cells (CTCs) are rare tumour cells found in the circulatory system of certain cancer patients. The clinical and functional significance of CTCs is still under investigation. Protein profiling of CTCs would complement the recent advances in enumeration, transcriptomic
Role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in DNA Repair.
Kosova A A, et al.
Biochemistry. Biokhimiia, 82(6), 643-654 (2017)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) aggregation causes mitochondrial dysfunction during oxidative stress-induced cell death.
Nakajima H, et al.
The Journal of Biological Chemistry, 292(11), 4727-4742 (2017)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service