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SAB4200024

Sigma-Aldrich

Anti-Notch 1 antibody, Rat monoclonal

clone ICN 5B5, purified from hybridoma cell culture

Synonym(s):

Anti-Notch homolog 1, translocation-associated (Drosophila), Anti-TAN1, hN1, neurogenic locus notch homolog protein 1, Anti-Translocation-associated notch protein, Monoclonal Anti-Notch 1 antibody produced in rat

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

ICN 5B5, monoclonal

form

buffered aqueous solution

mol wt

antigen ~120 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

western blot: 2-4 μg/mL using whole extract of human Jurkat cells and developing with a sensitive film

isotype

IgG2b

UniProt accession no.

P46531

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOTCH1(4851)

General description

Monoclonal Anti-Notch 1 (rat IgG2b isotype) is derived from the hybridoma ICN 5B5 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a recombinant fusion protein corresponding to a fragment of human Notch 1 fusion protein. Notch receptor 1 (Notch1) gene is located on human chromosome 9q34.

Application

Monoclonal Anti-Notch 1 antibody produced in rat has been used in immunoblotting.

Biochem/physiol Actions

In chronic obstructive pulmonary disease, notch receptor 1 (Notch1) helps to modulate endothelial apoptosis via the extracellular signal-regulated kinase (ERK) pathway. Overexpression of Notch1 helps to guard endothelial cell from apoptosis. Knockdown of Notch1 results in the enhancement of apoptotic cells. Notch1 serves as a tumor suppressor gene in mammalian epidermis and is a determinant of keratinocyte differentiation. Notch signaling is believed to participate in several human neoplasms including T-cell acute lymphoblastic leukemia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Deep sequencing and SNP array analyses of pediatric T-cell acute lymphoblastic leukemia reveal NOTCH1 mutations in minor subclones and a high incidence of uniparental isodisomies affecting CDKN2A
Karrman K, et al.
Journal of Hematology & Oncology, 8(1), 42-42 (2015)
Regulation of Notch1 gene expression by p53 in epithelial cells
Yugawa T, et al.
Molecular and Cellular Biology, 27(10), 3732-3742 (2007)
Notch1 regulates endothelial apoptosis via the ERK pathway in chronic obstructive pulmonary disease
Zong D, et al.
American Journal of Physiology. Cell Physiology, 315(3), C330-C340 (2018)
Clinical and molecular characteristics of XPO1 mutations in patients with chronic lymphocytic leukemia
Jain P, et al.
American Journal of Hematology, 91(11), E478-E478 (2016)
Laura Asnaghi et al.
PloS one, 9(8), e105372-e105372 (2014-08-29)
The transcriptional response promoted by hypoxia-inducible factors has been associated with metastatic spread of uveal melanoma. We found expression of hypoxia-inducible factor 1α (HIF-1α) protein in well-vascularized tumor regions as well as in four cell lines grown in normoxia, thus
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