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SAB4200054

Sigma-Aldrich

Anti-DNA polymerase δ p50 antibody, Rat monoclonal

clone PDK 7B4, purified from hybridoma cell culture

Synonym(s):

Anti-POLD2, Anti-Polymerase (DNA directed), delta 2, regulatory subunit 50kDa, Monoclonal Anti-DNA polymerase δ p50 antibody produced in rat

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PDK 7B4, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa cell extracts

isotype

IgG2a

UniProt accession no.

P49005

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POLD2(5425)

Related Categories

General description

Monoclonal Anti-DNA polymerase δ p50 (rat IgG2a isotype) is derived from the hybridoma PDK 7B4 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p50. DNA polymerase δ consists of the proliferating cell nuclear antigen (PCNA), the multi-subunit replication factor C, and the 4 subunit DNA polymerase complex: DNA Polymerase δ 1, 2, 3, 4 (POLD1, POLD2, POLD3 and POLD4). The core DNA polymerase δ exists as a heterodimeric enzyme with subunits of 125 and 50 kDa.
The DNA polymerase δ 2 subunit (POLD2) gene, with 11 exons spanning 10kb of genomic DNA, is mapped to human chromosome 7. The gene encoding 50kDa protein is characterized with the G+C-rich 5′-flanking region and it lacks a typical TATA box. The encoded protein is one of the components of human DNA Pol δ complex.

Immunogen

human p50

Application

Monoclonal Anti-DNA polymerase δ p50 antibody has been used in
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • western blot analysis

Biochem/physiol Actions

DNA polymerase δ 2 subunit (POLD2) gene codes for a protein that participates in DNA replication and repair. In COS-7 cells, regulatory region of the hPOLD2 gene has ability to facilitate the expression of chloramphenicol acetyltransferase reporter gene. Phosphatase and tensin homolog (PTEN) tumor suppressor gene decrease the expression of POLD2 gene associated with ovarian carcinogenesis. This protein might also function as a potential prognostic biomarker in ovarian carcinoma. POLD2 takes part in polymeraseδ (Pol δ) assembly by coordinating simultaneously with all other three subunits of the enzyme.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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POLD2 and KSP37 (FGFBP2) correlate strongly with histology, stage and outcome in ovarian carcinomas.
Elgaaen BV
PLoS ONE, 5 (2010)
FF483-484 motif of human Pol? mediates its interaction with the POLD2 subunit of Pol? and contributes to DNA damage tolerance.
Baldeck N, et.al.
Nucleic Acids Research, 43, 2116-2125 (2015)
Human DNA polymerase delta is a pentameric holoenzyme with a dimeric p12 subunit
Khandagale P, et al.
Life science alliance, 2(2), e201900323-e201900323 (2019)
Characterization of the 5'-flanking region of the gene encoding the 50 kDa subunit of human DNA polymerase delta.
Perez A
Biochimica et Biophysica Acta, 1493, 231-236 (2000)
Cloning of the cDNAs for the small subunits of bovine and human DNA polymerase delta and chromosomal location of the human gene (POLD2).
Zhang J
Genomics, 29, 179-186 (1995)
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