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SAB4200085

Sigma-Aldrich

Monoclonal Anti-AGO2 antibody produced in rat

~1.5 mg/mL, clone 11A9, purified immunoglobulin

Synonym(s):

AGO2 Antibody - Monoclonal Anti-AGO2 antibody produced in rat, Ago2 Antibody, Anti-Argonaute 2, Anti-EIF2C2 eukaryotic translation initiation factor 2C, 2, Anti-Q10

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

11A9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~85 kDa

species reactivity

monkey, rat, human, mouse, bovine

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using HeLa cell extracts

isotype

IgG2a

UniProt accession no.

Q9UKV8

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AGO2(27161)
rat ... Ago2(59117)

Related Categories

General description

Monoclonal Anti-AGO2 (rat IgG2a isotype) is derived from the hybridoma 11A9 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a peptide corresponding to amino acids 1-15 of human AGO2. Monoclonal Anti-AGO2 recognizes human, monkey, bovine, rat and mouse AGO2. Argonaute 2 (Ago2) is encoded by the gene mapped to human chromosome 8q24.3. The encoded protein belongs to the Ago protein family. AGO2 is mainly localized on the diffuse cytoplasm and stress granules.

Application

Monoclonal Anti-AGO2 antibody produced in rat has been used in:
  • immunofluorescent localization
  • western blot analysis
  • immunoprecipitation.

Biochem/physiol Actions

Argonaute 2 (Ago2) plays a vital role in short interfering RNA-mediated gene silencing. The encoded protein is regulated at both the transcriptional and posttranslational level in human breast cancer cell lines. Ago2 and enhanced micro-RNA activity is involved in the tumorigenic progression of breast cancer cell lines. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC), head and neck squamous cell carcinoma patients.

Physical form

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

Cell lysates should not be boiled before loading onto SDS-PAGE, but only warmed to 60°C for 15 min. In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

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flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Argonaute2 promotes tumor metastasis by way of up-regulating focal adhesion kinase expression in hepatocellular carcinoma
Cheng N, et al.
Hepatology, 57, 1906-1918 (2013)
Terminal Duplex Stability and Nucleotide Identity Differentially Control siRNA Loading and Activity in RNA Interference
Angart PA, et al.
Nucleic Acid Therapeutics, 26, 309-317 (2016)
Ryan J Golden et al.
Nature, 542(7640), 197-202 (2017-01-24)
MicroRNAs (miRNAs) perform critical functions in normal physiology and disease by associating with Argonaute proteins and downregulating partially complementary messenger RNAs (mRNAs). Here we use clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) genome-wide loss-of-function screening
The Argonaute protein family
Hock J and Meister G
Genome Biology, 9 (2008)
Jessie A G L van Buggenum et al.
Scientific reports, 6, 22675-22675 (2016-03-08)
Immuno-PCR combines specific antibody-based protein detection with the sensitivity of PCR-based quantification through the use of antibody-DNA conjugates. The production of such conjugates depends on the availability of quick and efficient conjugation strategies for the two biomolecules. Here, we present
View All Related Papers

Articles

Ago RIP to Isolate microRNA and their Targets Using Imprint® RNA Immunoprecipitation Kit

Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.

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